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Single cells for forensic DNA analysis-from evidence material to test tube.
MedLine Citation:
PMID:  21198592     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The purpose of this project was to develop a method that, while providing morphological quality control, allows single cells to be obtained from the surfaces of various evidence materials and be made available for DNA analysis in cases where only small amounts of cell material are present or where only mixed traces are found. With the SteREO Lumar.V12 stereomicroscope and UV unit from Zeiss, it was possible to detect and assess single epithelial cells on the surfaces of various objects (e.g., glass, plastic, metal). A digitally operated micromanipulator developed by aura optik was used to lift a single cell from the surface of evidence material and to transfer it to a conventional PCR tube or to an AmpliGrid(®) from Advalytix. The actual lifting of the cells was performed with microglobes that acted as carriers. The microglobes were held with microtweezers and were transferred to the DNA analysis receptacles along with the adhering cells. In a next step, the PCR can be carried out in this receptacle without removing the microglobe. Our method allows a single cell to be isolated directly from evidence material and be made available for forensic DNA analysis.
Authors:
Simon Brück; Heidrun Evers; Frank Heidorn; Ute Müller; Roland Kilper; Marcel A Verhoff
Publication Detail:
Type:  Journal Article     Date:  2010-09-14
Journal Detail:
Title:  Journal of forensic sciences     Volume:  56     ISSN:  1556-4029     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  176-80     Citation Subset:  IM    
Copyright Information:
© 2010 American Academy of Forensic Sciences.
Affiliation:
Department of Legal Medicine, University of Gießen, Frankfurter Straße 58, D-35392 Gießen, Germany aura optik GmbH, Wildenbruchstraße 15, D-07745 Jena, Germany.
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