| Single cell time-resolved quorum responses reveal dependence on cell density and configuration. | |
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MedLine Citation:
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PMID: 21527637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial communication via quorum sensing has been extensively investigated in recent years. Bacteria communicate in a complex manner through the production, release, and reception of diffusible low molecular weight chemical signaling molecules. Much work has focused on understanding the basic mechanisms of quorum sensing. As more and more bacteria grow resistant to conventional antibiotics, the development of drugs that do not kill bacteria but instead interrupt their communication is of increasing interest. This study presents a method for analyzing bacterial communication by investigating single cell responses. Most conventional analysis methods for bacterial communication are based on the averaged response from many bacteria, masking how individual cells respond to their immediate environment. We applied a fiber-optic microarray to record cellular communication from single cells. Single cell quorum sensing systems have previously been employed, but the highly ordered array reported here is an improvement because it allows us to simultaneously investigate cellular communication in many different environments with known cellular densities and configurations. We employed this method to detect how genes under quorum regulation are induced or repressed over time on the single cell level and to determine whether cellular density and configuration are indicative of the single cell temporal patterns of gene expression. |
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Authors:
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Ragnhild D Whitaker; Steven Pember; Byron C Wallace; Carla E Brodley; David R Walt |
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Publication Detail:
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Type: Journal Article Date: 2011-04-28 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-13 Completed Date: 2011-08-30 Revised Date: 2012-09-18 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 21623-32 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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metabolism Biophysics / methods Cell Communication Escherichia coli / metabolism Fiber Optic Technology Gene Expression Regulation, Bacterial* Models, Biological Models, Chemical Oligonucleotide Array Sequence Analysis Quorum Sensing / physiology* Time Factors Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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