Document Detail

Single-cell quantitative analysis of DNA incorporation and protein expression in microwells.
MedLine Citation:
PMID:  20214610     Owner:  NLM     Status:  MEDLINE    
Conventional biochemical assays are performed via an averaging procedure with the lysate of a large number of target cells; however, the averaged data lose information regarding the heterogeneity of individual cells. For quantitative assay of single cells, it is necessary to isolate single cells, extract cellular components and detect extremely small amounts of molecules from the individual cells. We developed new system combining a microfabricated lab-on-chip device and fluorescence correlation spectroscopy. The system features a simple protocol to isolate single-cells and detect small amounts of specific fluorescent molecules extracted from a single cell. Our results indicate that numbers of transfected DNA molecules were rather equal for each single cell; however, the expression rate of protein varied in each single cell.
Akira Sasaki; Hiroshi Sakata; Masataka Kinjo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current pharmaceutical biotechnology     Volume:  11     ISSN:  1873-4316     ISO Abbreviation:  Curr Pharm Biotechnol     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-03-10     Completed Date:  2010-06-17     Revised Date:  2010-08-31    
Medline Journal Info:
Nlm Unique ID:  100960530     Medline TA:  Curr Pharm Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  117-21     Citation Subset:  IM    
Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, Kita 21, Nishi 11, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan.
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MeSH Terms
Cell Separation / instrumentation*
Equipment Design
Equipment Failure Analysis
Gene Expression Profiling / instrumentation*
Microfluidic Analytical Techniques / instrumentation*
Oligonucleotide Array Sequence Analysis / instrumentation*
Protein Array Analysis / instrumentation*
Spectrometry, Fluorescence / instrumentation*

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