Document Detail

Single-cell printer: automated, on demand, and label free.
MedLine Citation:
PMID:  24222537     Owner:  NLM     Status:  In-Data-Review    
Within the past years, single-cell analysis has developed into a key topic in cell biology to study cellular functions that are not accessible by investigation of larger cell populations. Engineering approaches aiming to access single cells to extract information about their physiology, phenotype, and genotype at the single-cell level are going manifold ways, meanwhile allowing separation, sorting, culturing, and analysis of individual cells. Based on our earlier research toward inkjet-like printing of single cells, this article presents further characterization results obtained with a fully automated prototype instrument for printing of single living cells in a noncontact inkjet-like manner. The presented technology is based on a transparent microfluidic drop-on-demand dispenser chip coupled with a camera-assisted automatic detection system. Cells inside the chip are detected and classified with this detection system before they are expelled from the nozzle confined in microdroplets, thus enabling a "one cell per droplet" printing mode. To demonstrate the prototype instrument's suitability for biological and biomedical applications, basic experiments such as printing of single-bead and cell arrays as well as deposition and culture of single cells in microwell plates are presented. Printing efficiencies greater than 80% and viability rates about 90% were achieved.
Andre Gross; Jonas Schöndube; Sonja Niekrawitz; Wolfgang Streule; Lutz Riegger; Roland Zengerle; Peter Koltay
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of laboratory automation     Volume:  18     ISSN:  2211-0682     ISO Abbreviation:  J Lab Autom     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101558509     Medline TA:  J Lab Autom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504-18     Citation Subset:  IM    
1Laboratory for MEMS Applications, Department of Microsystems Engineering (IMTEK), University of Freiburg, Freiburg, Germany.
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