| Single cell polymerase chain reaction for preimplantation genetic diagnosis: methods, strategies, and limitations. | |
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MedLine Citation:
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PMID: 17876074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We provide an overview of the methodology involved in single cell polymerase chain reaction (PCR), especially for single lymphocytes or cultured lymphoblasts and blastomeres. The first step toward single cell PCR is isolation of single cells; the protocols given can be carried out using basic instruments such as a stereomicroscope. We also describe the alkaline lysis method for cell lysis as well as the design and execution of single cell PCR, either in simplex or in multiplex. Finally, we briefly discuss the different methods for analyzing PCR products and obtaining an accurate diagnosis. Special attention is given to measures that avoid contamination with extraneous DNA and reduce allele dropout. |
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Authors:
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Karen Sermon; Martine De Rycke |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular medicine Volume: 132 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-09-18 Completed Date: 2007-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 31-42 Citation Subset: IM |
Affiliation:
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Centre for Medical Genetics, Medical School of the Dutch-Speaking Brussels Free University (Vrije Universiteit Brussel), Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Blastomeres / chemistry*, cytology DNA / analysis* Female Humans Lymphocytes / chemistry*, cytology Polymerase Chain Reaction / methods* Preimplantation Diagnosis / methods* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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