Document Detail


Single cell polymerase chain reaction for preimplantation genetic diagnosis: methods, strategies, and limitations.
MedLine Citation:
PMID:  17876074     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We provide an overview of the methodology involved in single cell polymerase chain reaction (PCR), especially for single lymphocytes or cultured lymphoblasts and blastomeres. The first step toward single cell PCR is isolation of single cells; the protocols given can be carried out using basic instruments such as a stereomicroscope. We also describe the alkaline lysis method for cell lysis as well as the design and execution of single cell PCR, either in simplex or in multiplex. Finally, we briefly discuss the different methods for analyzing PCR products and obtaining an accurate diagnosis. Special attention is given to measures that avoid contamination with extraneous DNA and reduce allele dropout.
Authors:
Karen Sermon; Martine De Rycke
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  132     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2007  
Date Detail:
Created Date:  2007-09-18     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31-42     Citation Subset:  IM    
Affiliation:
Centre for Medical Genetics, Medical School of the Dutch-Speaking Brussels Free University (Vrije Universiteit Brussel), Belgium.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Blastomeres / chemistry*,  cytology
DNA / analysis*
Female
Humans
Lymphocytes / chemistry*,  cytology
Polymerase Chain Reaction / methods*
Preimplantation Diagnosis / methods*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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