Document Detail


Single cell methods for methane oxidation analysis.
MedLine Citation:
PMID:  21419920     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Respiration is widely used for evaluation of the metabolic capabilities or physiological state of the microbial culture. This chapter describes novel approaches for characterization of respiration at a single cell level: (1) flow cytometry-based redox sensing (FCRS) of actively metabolizing microbes; (2) respiration response imaging (RRI) for real-time detection of substrate stimulated redox responses of individual cells; (3) respiration detection system: microobservation chamber (RDS: MC), a single cell analysis system for carrying out the physiological and genomic profiling of cells capable of respiring C(1) compounds. The techniques are suitable for description of physiological heterogeneity among cells in a single microbial population and could be used to characterize distribution of methylotrophic ability among microbial cells in the natural environmental samples.
Authors:
Michael C Konopka; Sarah McQuaide; David S Ojala; Marina G Kalyuzhnaya; Mary E Lidstrom
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  495     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  149-66     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Department of Chemical Engineering, University of Washington, Seattle, Washington, USA.
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