| Single-cell mechanics provides a sensitive and quantitative means for probing amyloid-beta peptide and neuronal cell interactions. | |
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MedLine Citation:
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PMID: 20643929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By using a highly sensitive technique of atomic force microscopy-based single-cell compression, the rigidity of cultured N2a and HT22 neuronal cells was measured as a function of amyloid-beta42 (Abeta42) protein treatment. Abeta42 oligomers led to significant cellular stiffening; for example, 90-360% higher force was required to reach 80% deformation for N2a cells. Disaggregated or fibrillar forms of Abeta42 showed much less change. These observations were explained by a combination of two factors: (i) incorporation of oligomer into cellular membrane, which resulted in an increase in the Young's modulus of the membrane from 0.9+/-0.4 to 1.85+/-0.75 MPa for N2a cells and from 1.73+/-0.90 to 5.5+/-1.4 MPa for HT22 cells, and (ii) an increase in intracellular osmotic pressure (e.g., from 7 to 40 Pa for N2a cells) through unregulated ion influx. These findings and measurements provide a deeper, more characteristic, and quantitative insight into interactions between cells and Abeta42 oligomers, which have been considered the prime suspect for initiating neuronal dysfunction in Alzheimer's disease. |
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Authors:
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Valentin Lulevich; Christopher C Zimmer; Hyun-seok Hong; Lee-way Jin; Gang-yu Liu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-19 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-09-08 Revised Date: 2011-08-05 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 13872-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of California, Davis, CA 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Peptides
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chemistry* Animals Cell Communication* Cell Line Mice Neurons / chemistry*, physiology Nonlinear Dynamics Protein Multimerization |
| Grant Support | |
ID/Acronym/Agency:
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1R01 AG025500/AG/NIA NIH HHS; 1R21 AG031362/AG/NIA NIH HHS; 1R21 GM077850-01/GM/NIGMS NIH HHS; AG010129/AG/NIA NIH HHS; P30 AG010129-20/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Peptides |
| Comments/Corrections | |
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