Document Detail

Single-cell mechanics provides a sensitive and quantitative means for probing amyloid-beta peptide and neuronal cell interactions.
MedLine Citation:
PMID:  20643929     Owner:  NLM     Status:  MEDLINE    
By using a highly sensitive technique of atomic force microscopy-based single-cell compression, the rigidity of cultured N2a and HT22 neuronal cells was measured as a function of amyloid-beta42 (Abeta42) protein treatment. Abeta42 oligomers led to significant cellular stiffening; for example, 90-360% higher force was required to reach 80% deformation for N2a cells. Disaggregated or fibrillar forms of Abeta42 showed much less change. These observations were explained by a combination of two factors: (i) incorporation of oligomer into cellular membrane, which resulted in an increase in the Young's modulus of the membrane from 0.9+/-0.4 to 1.85+/-0.75 MPa for N2a cells and from 1.73+/-0.90 to 5.5+/-1.4 MPa for HT22 cells, and (ii) an increase in intracellular osmotic pressure (e.g., from 7 to 40 Pa for N2a cells) through unregulated ion influx. These findings and measurements provide a deeper, more characteristic, and quantitative insight into interactions between cells and Abeta42 oligomers, which have been considered the prime suspect for initiating neuronal dysfunction in Alzheimer's disease.
Valentin Lulevich; Christopher C Zimmer; Hyun-seok Hong; Lee-way Jin; Gang-yu Liu
Related Documents :
18831069 - Deletion of the amino-terminal domain of the prion protein does not impair prion protei...
15711059 - Fibroblast growth factor 2 is required for maintaining the neural stem cell pool in the...
19627439 - Nitric oxide and cgmp signal transduction positively regulates the motility of human ne...
3020189 - Differentiation and central projections of peripheral sensory cells with action-potenti...
9719419 - Establishment of an epidermal growth factor-dependent, multipotent neural precursor cel...
9671659 - Expression of kv1.1, a shaker-like potassium channel, is temporally regulated in embryo...
23519119 - Autophagy protein p62/sqstm1 is involved in hamlet-induced cell death by modulating apo...
1864339 - Orientation bias of cat dorsal lateral geniculate cells: directional analysis of the ma...
4020509 - Relationship between spatial-frequency and orientation tuning of striate-cortex cells.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-07-19
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-09-08     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13872-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amyloid beta-Peptides / chemistry*
Cell Communication*
Cell Line
Neurons / chemistry*,  physiology
Nonlinear Dynamics
Protein Multimerization
Grant Support
1R01 AG025500/AG/NIA NIH HHS; 1R21 AG031362/AG/NIA NIH HHS; 1R21 GM077850-01/GM/NIGMS NIH HHS; AG010129/AG/NIA NIH HHS; P30 AG010129/AG/NIA NIH HHS; P30 AG010129-20/AG/NIA NIH HHS; R21 GM077850/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Amyloid beta-Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Recovery of functional and structural age-related changes in the rat primary auditory cortex with op...
Next Document:  11-deoxycortisol is a corticosteroid hormone in the lamprey.