Document Detail

Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells.
MedLine Citation:
PMID:  21112354     Owner:  NLM     Status:  MEDLINE    
T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune synapse formation, as determined by LFA-1 and TCR enrichment at the interface of T-cell/APC conjugates using high throughput multispectral imaging flow cytometry. Together, these results suggest that ICAM-1/LFA-1 redistribution to the contact area is mainly responsible for development of strong interaction forces. High forces will keep T-cells and APCs in tight contact, thereby providing a platform for optimal interaction between TCRs and peptide-MHC complexes.
Sabrina Hoffmann; Babak H Hosseini; Markus Hecker; Ilia Louban; Nadja Bulbuc; Natalio Garbi; Guido H Wabnitz; Yvonne Samstag; Joachim P Spatz; Günter J Hämmerling
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-26
Journal Detail:
Title:  Immunology letters     Volume:  136     ISSN:  1879-0542     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-08     Completed Date:  2011-07-01     Revised Date:  2013-12-18    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  13-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Antigen-Presenting Cells / immunology*
Cell Line
Intercellular Adhesion Molecule-1 / immunology
Microscopy, Atomic Force
Myelin Sheath / immunology*
Peptides / immunology*
T-Lymphocytes / immunology*
Grant Support
Reg. No./Substance:
0/Peptides; 126547-89-5/Intercellular Adhesion Molecule-1

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