| Single-cell analysis of insulin-regulated fatty acid uptake in adipocytes. | |
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MedLine Citation:
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PMID: 20570821 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Increased body fat correlates with the enlargement of average fat cell size and reduced adipose tissue insulin sensitivity. It is currently unclear whether adipocytes, as they accumulate more triglycerides and grow in size, gradually become less insulin sensitive or whether obesity-related factors independently cause both the enlargement of adipocyte size and reduced adipose tissue insulin sensitivity. In the first instance, large and small adipocytes in the same tissue would exhibit differences in insulin sensitivity, whereas, in the second instance, adipocyte size per se would not necessarily correlate with insulin response. To analyze the effect of adipocyte size on insulin sensitivity, we employed a new single-cell imaging assay that resolves fatty acid uptake and insulin response in single adipocytes in subcutaneous adipose tissue explants. Here, we report that subcutaneous adipocytes are heterogeneous in size and intrinsic insulin sensitivity. Whereas smaller adipocytes respond to insulin by increasing lipid uptake, adipocytes with cell diameters larger than 80-100 microm are insulin resistant. We propose that, when cell size approaches a critical boundary, adipocytes lose insulin-dependent fatty acid transport. This negative feedback mechanism may protect adipocytes from lipid overload and restrict further expansion of adipose tissue, which leads to obesity and metabolic complications. |
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Authors:
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Oleg Varlamov; Romel Somwar; Anda Cornea; Paul Kievit; Kevin L Grove; Charles T Roberts |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-22 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 299 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-26 Completed Date: 2010-10-06 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E486-96 Citation Subset: IM |
Affiliation:
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Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, 97006, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology,
metabolism* Animals Biological Transport Blood Glucose / metabolism Boron Compounds / chemistry Cohort Studies Fatty Acids / metabolism* Female Image Processing, Computer-Assisted Insulin / blood, metabolism Insulin Resistance / physiology* Macaca mulatta Microscopy, Confocal Subcutaneous Fat / cytology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1-RO1-DK-79194/DK/NIDDK NIH HHS; P51 RR-00163/RR/NCRR NIH HHS; S10-RR-024585/RR/NCRR NIH HHS; //Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid; 0/Blood Glucose; 0/Boron Compounds; 0/Fatty Acids; 11061-68-0/Insulin |
| Comments/Corrections | |
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