Document Detail


Single-carbon catabolism in acetogens: analysis of carbon flow in Acetobacterium woodii and Butyribacterium methylotrophicum by fermentation and 13C nuclear magnetic resonance measurement.
MedLine Citation:
PMID:  6411684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The catabolism of methanol, formate, or carbon monoxide to acetate or butyrate or both was examined in two acetogenic bacteria. Butyribacterium methylotrophicum simultaneously transformed methanol and formate mainly to butyrate with concomitant H2 and CO2 production and consumption. In contrast, methanol plus CO was primarily converted to acetate, and only slight amounts of CO2 were produced. In vivo 13C nuclear magnetic resonance analysis of [13C]methanol transformation by B. methylotrophicum indicated that methanol was predominantly incorporated into the methyl of acetate. 13CO2 was produced and then consumed, and butyrate was formed from the condensation of two acetate precursors. The analysis of the position of acetate labeled by a given 13C single-carbon substrate when B. methylotrophicum or Acetobacterium woodii was grown in the presence of a second one-carbon substrate indicated two trends: when methanol was consumed, CO, CO2, or formate predominantly labeled the acetate carboxyl; when CO was consumed, CO2 and formate were principally funneled into the acetate methyl group, and CO remained a better carboxyl precursor. These data suggest a model of acetate synthesis via the combined operation of two readily reversible single-carbon pathways which are linked by CO2.
Authors:
R Kerby; W Niemczura; J G Zeikus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  155     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1208-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Carbon Dioxide / metabolism
Carbon Monoxide / metabolism*
Eubacterium / metabolism*
Formates / metabolism*
Formic Acids*
Gram-Positive Bacteria / metabolism*
Hydrogen / metabolism
Magnetic Resonance Spectroscopy
Methanol / metabolism*
Grant Support
ID/Acronym/Agency:
5 F32 AM06580/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Acetates; 0/Formates; 0/Formic Acids; 124-38-9/Carbon Dioxide; 1333-74-0/Hydrogen; 630-08-0/Carbon Monoxide; 64-18-6/formic acid; 67-56-1/Methanol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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