Document Detail

Single bioreactor gastrointestinal tract simulator for study of survival of probiotic bacteria.
MedLine Citation:
PMID:  18581109     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to design an in vitro model system to evaluate the probiotic potential of food. A single bioreactor system-gastrointestinal tract simulator (GITS) was chosen for process simulation on account of its considerable simplicity compared to multi-vessel systems used in previous studies. The bioreactor was evaluated by studying the viability of four known probiotic bacteria (Lactobacillus acidophilus La-5, Lactobacillus johnsonii NCC 533, Lactobacillus casei strain Shirota, and Lactobacillus rhamnosus GG) as a function of their physiological state. L. acidophilus and L. johnsonii survived in GITS better when introduced at an early stationary or exponential phase compared to being previously stored for 2 weeks at 4 degrees C. These two species were more resistant to bile salts and survived better than L. casei and L. rhamnosus GG. The latter two species gave large losses (up to 6 log) in plate counts independent of growth state due to the bile. However, experiments with some commercial probiotic products containing Lb. GG bacteria showed much better survival compared with model food (modified deMan-Rogosa-Sharpe growth medium), thus demonstrating the influence of the food matrix on the viability of bacteria. The study demonstrated that GITS can be successfully used for evaluation of viability of probiotic bacteria and functionality of probiotic food.
Ingrid Sumeri; Liisa Arike; Kaarel Adamberg; Toomas Paalme
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-26
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  80     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-10-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  317-24     Citation Subset:  IM    
Competence Centre of Food and Fermentation Technologies, Akadeemia tee 15b, 12618, Tallinn, Estonia.
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MeSH Terms
Bile Acids and Salts / metabolism*
Bioreactors / microbiology*
Dairy Products / analysis,  microbiology*
Food Microbiology*
Lactobacillus / growth & development*
Microbial Viability*
Probiotics / chemistry*
Reg. No./Substance:
0/Bile Acids and Salts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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