| Single-Tube Nested Real-Time PCR as a New Highly Sensitive Approach to Trace Hazelnut. | |
| | |
MedLine Citation:
|
PMID: 22849792 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Hazelnut is one of the most commonly consumed tree nuts, being largely used by the food industry in a wide variety of processed foods. However, it is a source of allergens capable of inducing mild to severe allergic reactions in sensitized individuals. Hence, the development of highly sensitive methodologies for hazelnut traceability is essential. In this work, we developed a novel technique for hazelnut detection based on single-tube nested real-time PCR system. The system presents high specificity and sensitivity, enabling a relative limit of detection of 50 mg/kg of hazelnut in wheat material and an absolute limit of detection of 0.5 pg of hazelnut DNA (1 DNA copy). Its application to processed food samples was successfully achieved, detecting trace amounts of hazelnut in chocolate down to 60 mg/kg. These results highlight the adequacy of the technique for the specific detection and semi-quantitation of hazelnut as potential hidden allergens in foods. |
| | |
Authors:
|
Joana Costa; Isabel Mafra; Tomas Kuchta; M Beatriz P P Oliveira |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-7-31 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-8-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Postmenopausal skin and estrogen.
Next Document: Impact of central nervous system (CNS) prophylaxis on the incidence and risk factors for CNS relapse...