Document Detail

Single-Layer Centrifugation Reduces Equine Arteritis Virus Titre in the Semen of Shedding Stallions.
MedLine Citation:
PMID:  23190015     Owner:  NLM     Status:  Publisher    
Several countries have adopted strategies for preventing and/or controlling equine viral arteritis based on vaccination and restricting the breeding activities of carrier stallions. However, in some cases, carrier stallions are only identified after they have transmitted virus to a mare. Therefore, a mechanism for separating virus from spermatozoa in the semen of carrier stallions would facilitate control measures for preventing disease transmission. In this study, the use of several modifications of single-layer centrifugation (SLC, SLC with an inner tube and double SLC) through Androcoll-E, a species-specific colloid were evaluated for their ability to separate spermatozoa from virus in ejaculates from carrier stallions. The three types of SLC significantly reduced the virus titre in fresh semen at 0 h and in stored semen at 24 h (p < 0.001) but did not completely eliminate the virus. Sperm motility parameters such as total motility and progressive motility were significantly increased after colloid centrifugation, whereas curvilinear velocity and amplitude of lateral head deviation were decreased, and the remainder (straight line velocity, average path velocity, straightness, linearity, wobble and beat cross-frequency) were not significantly affected by the processing. Although virus titres were reduced in the SLC samples, significant levels of infectivity still remained, especially in stallions shedding large amounts of virus. It remains to be determined whether SLC-processed sperm samples from stallions shedding low virus titres retain sufficient equine arteritis virus to cause infection in mares through artificial insemination.
Jm Morrell; P Timoney; C Klein; K Shuck; J Campos; M Troedsson
Related Documents :
23085775 - Collinearity of protease mutations in hiv-1 samples with high-level protease inhibitor ...
24503085 - Repair of the 3' proximal and internal deletions of a satellite rna associated with cuc...
23908945 - Tracking oseltamivir-resistance in new zealand influenza viruses during a medicine recl...
23814385 - Herpes simplex virus meningitis complicated by ascending paralysis.
1328555 - Effect of biological and synthetic polymers on bk virus infectivity and hemagglutination.
25354585 - Aptamer-based biosensors for the rapid visual detection of flu viruses.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  -     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Verlag GmbH.
Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Isolated Congenital Left Ventricular Diverticulum in an Adult.
Next Document:  Incidence and Predictors of Radial Artery Spasm at the Beginning of a Transradial Coronary Procedure...