Document Detail

Single-cell lipidomics: characterizing and imaging lipids on the surface of individual Aplysia californica neurons with cluster secondary ion mass spectrometry.
MedLine Citation:
PMID:  23323749     Owner:  NLM     Status:  MEDLINE    
Neurons isolated from Aplysia californica , an organism with a well-defined neural network, were imaged with secondary ion mass spectrometry, C(60)-SIMS. A major lipid component of the neuronal membrane was identified as 1-hexadecyl-2-octadecenoyl-sn-glycero-3-phosphocholine [PC(16:0e/18:1)] using tandem mass spectrometry (MS/MS). The assignment was made directly off the sample surface using a C(60)-QSTAR instrument, a prototype instrument that combines an ion source with a commercial electrospray ionization/matrix-assisted laser desorption ionization (ESI/MALDI) mass spectrometer. Normal phase liquid chromatography mass spectrometry (NP-LC-MS) was used to confirm the assignment. Cholesterol and vitamin E were also identified with in situ tandem MS analyses that were compared to reference spectra obtained from purified compounds. In order to improve sensitivity on the single-cell level, the tandem MS spectrum of vitamin E reference material was used to extract and compile all the vitamin E related peaks from the cell image. The mass spectrometry images reveal heterogeneous distributions of intact lipid species, PC(16:0e/18:1), vitamin E, and cholesterol on the surface of a single neuron. The ability to detect these molecules and determine their relative distribution on the single-cell level shows that the C(60)-QSTAR is a potential platform for studying important biochemical processes, such as neuron degeneration.
Melissa K Passarelli; Andrew G Ewing; Nicholas Winograd
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-04
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-19     Completed Date:  2013-11-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2231-8     Citation Subset:  IM    
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MeSH Terms
Aplysia / metabolism*
Cholesterol / analysis
Lipids / analysis*
Neurons / cytology,  metabolism*
Phosphatidylcholines / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrometry, Mass, Secondary Ion*
Tandem Mass Spectrometry
Vitamin E / analysis
Grant Support
2R01 EB002016-18/EB/NIBIB NIH HHS; GM069338-07/GM/NIGMS NIH HHS; R01 EB002016/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Lipids; 0/Phosphatidylcholines; 1406-18-4/Vitamin E; 97C5T2UQ7J/Cholesterol

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