Document Detail


Single-cell lipidomics: characterizing and imaging lipids on the surface of individual Aplysia californica neurons with cluster secondary ion mass spectrometry.
MedLine Citation:
PMID:  23323749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurons isolated from Aplysia californica , an organism with a well-defined neural network, were imaged with secondary ion mass spectrometry, C(60)-SIMS. A major lipid component of the neuronal membrane was identified as 1-hexadecyl-2-octadecenoyl-sn-glycero-3-phosphocholine [PC(16:0e/18:1)] using tandem mass spectrometry (MS/MS). The assignment was made directly off the sample surface using a C(60)-QSTAR instrument, a prototype instrument that combines an ion source with a commercial electrospray ionization/matrix-assisted laser desorption ionization (ESI/MALDI) mass spectrometer. Normal phase liquid chromatography mass spectrometry (NP-LC-MS) was used to confirm the assignment. Cholesterol and vitamin E were also identified with in situ tandem MS analyses that were compared to reference spectra obtained from purified compounds. In order to improve sensitivity on the single-cell level, the tandem MS spectrum of vitamin E reference material was used to extract and compile all the vitamin E related peaks from the cell image. The mass spectrometry images reveal heterogeneous distributions of intact lipid species, PC(16:0e/18:1), vitamin E, and cholesterol on the surface of a single neuron. The ability to detect these molecules and determine their relative distribution on the single-cell level shows that the C(60)-QSTAR is a potential platform for studying important biochemical processes, such as neuron degeneration.
Authors:
Melissa K Passarelli; Andrew G Ewing; Nicholas Winograd
Related Documents :
24036499 - Simultaneous determination of olmesartan and amlodipine in human plasma and urine by ul...
24276279 - Gas chromatographic-mass spectroscopic identification of protaglandin f2α in flowering...
11101249 - A study of the enthalpy and entropy contributions of the stationary phase in reversed-p...
24167149 - Dart-ms for rapid, preliminary screening of urine for dmaa.
18064619 - Prediction of elution bandwidth for purine compounds by a retention model in reversed-p...
21067759 - Physicochemical characterization of dilute n-alcohol/biodiesel mixtures by inverse gas ...
23223379 - A facile versatile polymeric monolith for multiple separations.
8346649 - Chemical assay of avermectins by high performance liquid chromatography with fluorescen...
19349169 - Generation of high-purity hydrogen from cellulose by its mechanochemical treatment.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-04
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-19     Completed Date:  2013-11-18     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2231-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Aplysia / metabolism*
Cholesterol / analysis
Lipids / analysis*
Neurons / cytology,  metabolism*
Phosphatidylcholines / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrometry, Mass, Secondary Ion*
Tandem Mass Spectrometry
Vitamin E / analysis
Grant Support
ID/Acronym/Agency:
2R01 EB002016-18/EB/NIBIB NIH HHS; GM069338-07/GM/NIGMS NIH HHS; R01 EB002016/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Lipids; 0/Phosphatidylcholines; 1406-18-4/Vitamin E; 97C5T2UQ7J/Cholesterol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Did We Interpret the Same Thing?
Next Document:  Specific and sensitive detection of Alcaligenes species from an agricultural environment.