Document Detail


Single-Cell Imaging Techniques for the Real-Time Detection of IP(3) in Live Cells.
MedLine Citation:
PMID:  23007586     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Inositol 1,4,5-trisphosphate (IP(3)) is a ubiquitous second messenger, derived from the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP(2)) by enzymes of the phospholipase C (PLC) family. Binding of IP(3) to its cognate receptor in the endoplasmic reticulum membrane leads to release of Ca(2+) into the cytoplasm, which is involved in the regulation of an array of cellular functions. Traditional techniques for the detection of IP(3) have required the extraction of a large number of cells, with limitations in the time resolution of changes in IP(3) and an inability to obtain detailed information on the dynamics of this second messenger in single cells. Recent progress in this field has led to the development of a number of genetically encoded fluorescent biosensors, which upon recombinant expression are able selectively to detect real-time changes in IP(3) in single live cells. In this chapter, I detail protocols for the expression, visualization (by confocol or fluorescence microscopy), and interpretation of data obtained with such biosensors expressed in mammalian cells.
Authors:
Carl P Nelson
Related Documents :
24658126 - Piwi is required in multiple cell types to control germline stem cell lineage developme...
24302766 - Incomplete cytokinesis and re-fusion of small mononucleated hodgkin cells lead to giant...
24780736 - Exploring the function of cell shape and size during mitosis.
24147526 - Characterization of the cells in repair tissue following autologous chondrocyte implant...
11976016 - A quantitative evaluation of radiolabelled lectin retention on oral mucosa in vitro and...
8647716 - Migration of hyaline cells into the chick basilar papilla during severe noise damage.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  937     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-92     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Sciences, Division of Anaesthesia, Critical Care, and Pain Management, Leicester Royal Infirmary, University of Leicester, Leicester, UK, cpn5@le.ac.uk.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Measurement of phospholipase C by monitoring inositol phosphates using [(3)h]inositol labeling proto...
Next Document:  Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay.