Document Detail


Single-Cell Copy Number Analysis of Prostate Cancer Cells Captured with GEDI Microdevices.
MedLine Citation:
PMID:  25363873     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Limited access to tumor tissue makes repeated sampling and real-time tracking of cancer progression infeasible. Circulating tumor cells (CTCs) provide the capacity for real-time genetic characterization of a disseminating tumor cell population via simple blood draw. However, there is no straightforward method to analyze broadscale genetic rearrangements in this heterogeneous cell population at the single cell level. We present a work for one-step controllable chemical extraction of whole nuclei from prostate cancer cells captured using GEDI microdevices. We have successfully used copy number profile analysis to differentiate between two unique cancer cell line populations of metastatic origin (LNCaP and VCaP), and to analyze key mutations important in disease progression.
Authors:
Erica D Pratt; Asya Stepansky; James Hicks; Brian J Kirby
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-1
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-3     Completed Date:  -     Revised Date:  2014-11-5    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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