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Single Cell Confocal Spectrometry of a Filamentous Cyanobacterium Nostoc at room and cryogenic temperature. Diversity and Differentiation of Pigment Systems in 311 Cells.
MedLine Citation:
PMID:  22739509     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
1) Fluorescence spectrum at 298 and 40 K and absorption spectrum at 298 K of each cyanobacterial cell was measured by the single-cell confocal laser spectroscopy to study differentiation of cell pigments. 2) Fluorescence spectra of vegetative ( veg) and heterocyst ( het) cells of a filamentous cyanobacterium Nostoc sp. formed separate groups with low and high photosystem II to I (PSII/PSI) ratios, respectively. 3) The fluorescence spectra of het cells at 40 K still contained typical PSII bands. 4) PSII/PSI ratio estimated for the veg cells varied at 0.4-1.2, while that of het cells at 0-0.22 even in the same culture. 5) The PSII/PSI ratio of veg cells resembled more closely each other in the same filament. 6) " pro-het" cells, which started to differentiate into het cells, were identified from the small but specific difference of PSII/PSI ratio. 7) Allophycocyanin (APC)/PSII ratio was almost constant both in veg and het cells indicating their tight couplings. 8) Phycocyanin (PC) showed higher fluorescence in most of het cells suggesting the uncoupling from PSII. 9) Veg cells seems to vary PSI contents to give different PSII/PSI ratio even in the same culture, and to suppress the synthesis of PSII, APC and PC to differentiate into het cells. APC and PC are gradually liberated from membranes in het cells as the uncoupling from PSII. 10) Single cell spectrometry will be useful to study the differentiation of intrinsic pigments of cells and chloroplasts, and to select microbes from natural environments.
Authors:
Kana Sugiura; Shigeru Itoh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-26
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Division of Material Science Physics, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
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