| Simvastatin suppresses homocysteine-induced apoptosis in endothelial cells: roles of caspase-3, cIAP-1 and cIAP-2. | |
| | |
MedLine Citation:
|
PMID: 19343037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The present study addresses how homocysteine (Hcy) induces endothelial apoptosis and how simvastatin antagonizes these pro-apoptotic effects of Hcy.Human umbilical vein endothelial cells (HUVECs) were treated with Hcy for 24 h, in the presence or absence of simvastatin. Cell apoptosis was evaluated by Annexin V staining and flow cytometry, as well as transferase-mediated dUTP-biotin nick end labeling (TUNEL). The mRNA and protein levels of caspase-3, cellular inhibitor of apoptosis (cIAP)-1 and -2 were analyzed by real-time polymerase chain reaction (RT-PCR) and western blotting, respectively.Treatment with both low (0.05 mmol l(-1)) and high (0.3 mmol l(-1)) concentrations of Hcy induced apoptosis in HUVECs which was accompanied by an increased level of caspase-3 expression and activation, together with decreased cIAP-1 and cIAP-2 levels. Conversely, simvastatin upregulated c-IAP1 and c-IAP2 expression, while attenuating Hcy-induced apoptosis and caspase-3 activation. Hcy may induce HUVEC apoptosis through a pathway involving caspase-3. This induction can be partially antagonized by simvastatin, possibly through upregulation of cIAP-1 and cIAP-2. |
| | |
Authors:
|
Zhihong Xu; Guoping Lu; Fang Wu |
Related Documents
:
|
9200447 - Bcl-2 expression in target cells leads to functional inhibition of caspase-3 protease f... 11408917 - Activation of caspase-3 in renal cell carcinoma cells by anthracyclines or 5-fluorouracil. 11854437 - Caspase-dependent cleavage of carbamoyl phosphate synthetase ii during apoptosis. 16988947 - Mechanism of apoptosis with the involvement of calpain and caspase cascades in human ma... 21464967 - Modulation of human immune responses by bovine interleukin-10. 14726697 - Synergy between pi3k/akt and raf/mek/erk pathways in igf-1r mediated cell cycle progres... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-03 |
Journal Detail:
|
Title: Hypertension research : official journal of the Japanese Society of Hypertension Volume: 32 ISSN: 1348-4214 ISO Abbreviation: Hypertens. Res. Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-05-06 Completed Date: 2009-11-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9307690 Medline TA: Hypertens Res Country: Japan |
Other Details:
|
Languages: eng Pagination: 375-80 Citation Subset: IM |
Affiliation:
|
Department of Geriatrics, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Caspase 3 / genetics, metabolism Cells, Cultured Endothelium, Vascular / drug effects*, metabolism Homocysteine / antagonists & inhibitors*, metabolism, pharmacology Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology* Hyperhomocysteinemia / metabolism Inhibitor of Apoptosis Proteins / genetics, metabolism Simvastatin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 0/Inhibitor of Apoptosis Proteins; 454-28-4/Homocysteine; 79902-63-9/Simvastatin; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The taste of salt measured by a simple test and blood pressure in Japanese women and men.
Next Document: Malignant pleural mesothelioma cells resist anoikis as quiescent pluricellular aggregates.