Document Detail


Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species.
MedLine Citation:
PMID:  17242468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After immunohistochemistry (IHC) began to be used routinely, a number of investigators worked on methods for staining multiple molecules in the same tissue sections or cells. Achieving this goal was not easy, however. One reason for this is that the majority of primary antibodies used in IHC reactions are raised in rabbits, and recognizing signals from two different rabbit antibodies is not trivial. Thus, all of the protocols described to date have serious limitations. Here we report a simple, quick, and inexpensive solution to the problem. It has two major advantages over existing methods. First, by using antibodies from the same host, two or more antigens can be visualized in the same section with commercially available fluorescent dyes. Second, because the technique relies on signal amplification, both rare and abundant antigens can be detected.
Authors:
Zsuzsanna E Tóth; Eva Mezey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2007-01-22
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  55     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  545-54     Citation Subset:  IM    
Affiliation:
Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Researh, National Institutes of Health, Bethesda, MD 20892-2190, USA. tothzs@ana.sote.hu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / chemistry,  immunology*
Antibody Specificity / immunology
Antigens / analysis,  immunology*
Cross Reactions / immunology
Fluorescein-5-isothiocyanate / chemistry
Fluorescent Dyes / chemistry
Glial Fibrillary Acidic Protein / analysis,  immunology
Goats
Immunoglobulin G / immunology
Immunohistochemistry / methods*
Male
Mice
Microwaves
Peroxidase / chemistry
Rabbits
Rats
Reproducibility of Results
Species Specificity
Tyramine / chemistry*
Tyrosine 3-Monooxygenase / analysis,  immunology
Vasopressins / analysis,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens; 0/Fluorescent Dyes; 0/Glial Fibrillary Acidic Protein; 0/Immunoglobulin G; 11000-17-2/Vasopressins; 3326-32-7/Fluorescein-5-isothiocyanate; 51-67-2/Tyramine; EC 1.11.1.7/Peroxidase; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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