Document Detail

Simultaneous serum desalting and total protein determination by macroporous reversed-phase chromatography.
MedLine Citation:
PMID:  23388688     Owner:  NLM     Status:  Publisher    
Macroporous reversed-phase (mRP) chromatography was successfully used to develop an accurate and precise method for total protein in serum. The limits of detection (0.83 μg, LOD) and quantification (2.51 μg, LOQ) for the mRP method are comparable with those of the widely used micro BCA protein assay. The mRP method can be used to determine the total protein concentration across a wide dynamic range by detecting chromatographic peaks at 215 nm and 280 nm. The method has the added advantage of desalting and denaturing proteins, leading to more complete digestion by trypsin and to better LC-MS-MS identification in shotgun proteomics experiments.
Alexander Boichenko; Natalia Govorukhina; Ate G J van der Zee; Rainer Bischoff
Related Documents :
23578308 - A novel approach for the quantitation of carbohydrates in mash, wort, and beer with rp-...
24530708 - Simultaneous spectrophotometric determination of celecoxib and diacerein in bulk and ca...
23724218 - Evaluation of three methods in the diagnosis of dentin cracks caused by apical resection.
23378098 - Laser desorption dual spray post-ionization mass spectrometry for direct analysis of sa...
1491038 - Determination of acetazolamide in human urine samples by reversed-phase high-performanc...
21644778 - Viscoelastic properties of low-viscosity liquids studied with thickness-shear mode reso...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-7
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Analytical Biochemistry, University of Groningen, 9713 AV, Groningen, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Proactive Treatment of Adult Facial Seborrhoeic Dermatitis with 0.1% Tacrolimus Ointment: Randomized...
Next Document:  A novel homogeneous immunoassay for anthrax detection based on the AlphaLISA method: detection of B....