| Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes. | |
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MedLine Citation:
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PMID: 17654594 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular/extracellular lactate (Lac) distribution has been determined before in human and animal erythrocytes (red blood cells [RBCs]) with various methods. However, all previous methods determine intra- and extracellular Lac separately or indirectly. Now, (13)C-NMR spectroscopy has been used to monitor intra- and extracellular Lac simultaneously in intact RBCs. Isolated human RBCs were incubated with [3-(13)C]-Lac, [3-(13)C]-pyruvate (Pyr), and [1-(13)C]-glucose (Gluc). A distortionless enhancement by polarization transfer (DEPT) sequence was used (TR = 3.3 s, N = 128) to monitor the (13)C-NMR resonances in both compartments. The intra- and extracellular methyl group resonances of Lac and Pyr were clearly separated by 9.6 Hz and 7.0 Hz, respectively, under normoxic conditions due to the RBC chemical-shift effect. The results show that the chemical-shift effect of RBCs is convenient to monitor intra- and extracellular Lac simultaneously in intact RBCs under normoxic conditions. |
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Authors:
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Götz Kohler; Florian Rost; Joachim Seelig |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 58 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-30 Completed Date: 2007-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
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Languages: eng Pagination: 213-7 Citation Subset: IM |
Affiliation:
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Biozentrum, Department of Biophysical Chemistry, University of Basel, Basel, Switzerland. goetz.kohler@unibas.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blood Glucose
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metabolism Carbon Isotopes Erythrocytes / chemistry*, metabolism Extracellular Space / metabolism Humans Intracellular Space / metabolism Lactates / metabolism* Magnetic Resonance Spectroscopy / methods* Pyruvic Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Carbon Isotopes; 0/Lactates; 127-17-3/Pyruvic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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