| Simultaneous saccharification and fermentation of acid-pretreated rapeseed meal for succinic acid production using Actinobacillus succinogenes. | |
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MedLine Citation:
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PMID: 22112947 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Rapeseed meal was evaluated for succinic acid production by simultaneous saccharification and fermentation using Actinobacillus succinogenes ATCC 55618. Diluted sulfuric acid pretreatment and subsequent hydrolysis with pectinase was used to release sugars from rapeseed meal. The effects of culture pH, pectinase loading and yeast extract concentration on succinic acid production were investigated. When simultaneous saccharification and fermentation of diluted acid pretreated rapeseed meal with a dry matter content of 12.5% (w/v) was performed at pH 6.4 and a pectinase loading of 2% (w/w, on dry matter) without supplementation of yeast extract, a succinic acid concentration of 15.5g/L was obtained at a yield of 12.4g/100g dry matter. Fed-batch simultaneous saccharification and fermentation was carried out with supplementation of concentrated pretreated rapeseed meal and pectinase at 18 and 28h to yield a final dry matter content of 20.5% and pectinase loading of 2%, with the succinic acid concentration enhanced to 23.4g/L at a yield of 11.5g/100g dry matter and a productivity of 0.33g/(Lh). This study suggests that rapeseed meal may be an alternative substrate for the efficient production of succinic acid by A. succinogenes without requiring nitrogen source supplementation. |
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Authors:
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Kequan Chen; Han Zhang; Yelian Miao; Ping Wei; Jieyu Chen |
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Publication Detail:
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Type: Journal Article Date: 2010-12-23 |
Journal Detail:
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Title: Enzyme and microbial technology Volume: 48 ISSN: 0141-0229 ISO Abbreviation: Enzyme Microb. Technol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003761 Medline TA: Enzyme Microb Technol Country: United States |
Other Details:
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Languages: eng Pagination: 339-44 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Faculty of Bioresource Sciences, Akita Prefectural University, Kaidobata-Nishi 241-438, Shimoshinjo-Nakano, Akita-shi, Akita 010-0195, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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