Document Detail


Simultaneous retention index analysis of urinary amino acids and carboxylic acids for graphic recognition of abnormal state.
MedLine Citation:
PMID:  15894518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Simultaneous profiling analysis of urinary amino acids (AAs) and carboxylic acids (CAs) was combined with retention index (I) analysis for graphic recognition of abnormal metabolic state. The temperature-programmed I values of the AA and CA standards measured as ethoxycarbonyl (EOC)/methoxime (MO)/tert-butyldimethylsilyl (TBDMS) derivatives were used as the reference I values. Urine samples were subjected to the sequential EOC, MO and TBDMS reactions for the analysis by gas chromatography (GC) and GC-mass spectrometry. The complex GC profiles were then transformed into their respective I patterns in bar graphic forms by plotting the normalized peak area ratios (%) of the identified AAs and CAs against their reference I values as the identification numbers. When the present method was applied to infant urine specimens from normal controls and patients with inherited metabolic diseases such as phenylketonuria, maple syrup urine disease, methylmalonic aciduria or isovaleric aciduria, each I pattern of bar graph more distinctly displayed quantitative abundances of urinary AAs and CAs in qualitative I scale, thus allowing graphic discrimination between normal and abnormal states.
Authors:
Man-Jeong Paik; Hong-Jin Lee; Kyoung-Rae Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  821     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-01     Completed Date:  2005-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-104     Citation Subset:  IM    
Affiliation:
Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University, 300 Chunchun-dong, Suwon, Kyunggi-do 440-746, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / urine*
Carboxylic Acids / urine*
Child
Child, Preschool
Chromatography, Gas / methods*
Humans
Hydroxylamines / chemistry
Infant
Maple Syrup Urine Disease / urine
Metabolism, Inborn Errors / urine*
Methylmalonic Acid / urine
Organosilicon Compounds / chemistry
Pentanoic Acids / urine
Phenylketonurias / urine
Trifluoroacetic Acid / analogs & derivatives,  chemistry
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carboxylic Acids; 0/Hydroxylamines; 0/Organosilicon Compounds; 0/Pentanoic Acids; 503-74-2/isovaleric acid; 516-05-2/Methylmalonic Acid; 67-62-9/methoxyamine; 76-05-1/Trifluoroacetic Acid; 77377-52-7/N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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