| Simultaneous quantitative determination of deuterium- and carbon-13-labeled essential fatty acids in rat plasma. | |
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MedLine Citation:
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PMID: 15930513 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study reports methods for the quantitative determination of stable isotope-labeled essential fatty acids (EFAs) as well as an experiment in which deuterium-labeled linoleic acid (18:2n-6) and alpha-linolenic acid (18:3n-3) were compared with those labeled with carbon-13 in rat plasma in vivo. Standard curves were constructed to compensate for concentration and plasma matrix effects. It was observed that endogenous pools of fatty acids had a greater suppressing effect on the measurements of 13C-U-labeled EFAs relative to those labeled with 2H5. Using these methods, the in vivo metabolism of orally administered deuterated-linolenate, 13C-U-labeled linolenate, deuterated-linoleate, and 13C-U-labeled linoleate was compared in adult rats (n = 11). There were no significant differences in the concentrations of the 2H versus 13C isotopomers of 18:2n-6, 18:3n-3, arachidonic acid (20:4n-6), and docosahexaenoic acid (22:6n-3) in rat plasma samples at 24 h after dosing. Thus, there appears to be little isotope effect for 2H5- versus 13C-U-labeled EFAs when the data are calculated using the conventional standard curves and corrected for endogenous fatty acid pool size and matrix effects. |
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Authors:
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Yu Hong Lin; Robert J Pawlosky; Norman Salem |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Intramural Date: 2005-06-01 |
Journal Detail:
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Title: Journal of lipid research Volume: 46 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-16 Completed Date: 2005-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1974-82 Citation Subset: IM |
Affiliation:
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Section of Nutritional Neuroscience, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892-9410, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / blood Carbon Isotopes / diagnostic use Chromatography, Gas / methods Deuterium / diagnostic use Docosahexaenoic Acids / blood Fatty Acids, Essential / blood*, pharmacokinetics Female Gas Chromatography-Mass Spectrometry Isotope Labeling Linoleic Acid / blood Male Rats Rats, Sprague-Dawley alpha-Linolenic Acid / blood |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Fatty Acids, Essential; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 463-40-1/alpha-Linolenic Acid; 506-32-1/Arachidonic Acid; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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