Document Detail


Simultaneous quantitative determination of deuterium- and carbon-13-labeled essential fatty acids in rat plasma.
MedLine Citation:
PMID:  15930513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study reports methods for the quantitative determination of stable isotope-labeled essential fatty acids (EFAs) as well as an experiment in which deuterium-labeled linoleic acid (18:2n-6) and alpha-linolenic acid (18:3n-3) were compared with those labeled with carbon-13 in rat plasma in vivo. Standard curves were constructed to compensate for concentration and plasma matrix effects. It was observed that endogenous pools of fatty acids had a greater suppressing effect on the measurements of 13C-U-labeled EFAs relative to those labeled with 2H5. Using these methods, the in vivo metabolism of orally administered deuterated-linolenate, 13C-U-labeled linolenate, deuterated-linoleate, and 13C-U-labeled linoleate was compared in adult rats (n = 11). There were no significant differences in the concentrations of the 2H versus 13C isotopomers of 18:2n-6, 18:3n-3, arachidonic acid (20:4n-6), and docosahexaenoic acid (22:6n-3) in rat plasma samples at 24 h after dosing. Thus, there appears to be little isotope effect for 2H5- versus 13C-U-labeled EFAs when the data are calculated using the conventional standard curves and corrected for endogenous fatty acid pool size and matrix effects.
Authors:
Yu Hong Lin; Robert J Pawlosky; Norman Salem
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Intramural     Date:  2005-06-01
Journal Detail:
Title:  Journal of lipid research     Volume:  46     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-16     Completed Date:  2005-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1974-82     Citation Subset:  IM    
Affiliation:
Section of Nutritional Neuroscience, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892-9410, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / blood
Carbon Isotopes / diagnostic use
Chromatography, Gas / methods
Deuterium / diagnostic use
Docosahexaenoic Acids / blood
Fatty Acids, Essential / blood*,  pharmacokinetics
Female
Gas Chromatography-Mass Spectrometry
Isotope Labeling
Linoleic Acid / blood
Male
Rats
Rats, Sprague-Dawley
alpha-Linolenic Acid / blood
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids, Essential; 2197-37-7/Linoleic Acid; 25167-62-8/Docosahexaenoic Acids; 463-40-1/alpha-Linolenic Acid; 506-32-1/Arachidonic Acid; 7782-39-0/Deuterium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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