| Simultaneous quantitation of loxapine, amoxapine and their 7- and 8-hydroxy metabolites in plasma by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 1860915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Loxapine, its N-demethylated metabolite amoxapine, and their 7- and 8-hydroxy metabolites were determined simultaneously in plasma by a simple two-step extraction procedure followed by reversed-phase liquid chromatography. Baseline separation was achieved by a 5-microns Spherisorb C6 column. The mobile phase consisted of 5 mM phosphate buffer (with 14 mM orthophosphoric acid)-acetonitrile (with 105 microM nonylamine) (77:23, v/v). Assays of the steady-state plasma samples obtained from seventeen patients on loxapine showed substantial amounts of 8-hydroxy metabolites, lesser amounts of loxapine, amoxapine and 7-hydroxyloxapine and trace amounts of 7-hydroxyamoxapine. As 8-hydroxy metabolites possess only weak dopamine-D2 blocking activity, the final neuroleptic property of loxapine may be affected significantly by metabolic polymorphism. |
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Authors:
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S W Cheung; S W Tang; G Remington |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 564 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-08-30 Completed Date: 1991-08-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 213-21 Citation Subset: IM |
Affiliation:
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Psychopharmacology Unit, Clarke Institute of Psychiatry, Toronto, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amoxapine
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blood* Animals Binding, Competitive Chromatography, High Pressure Liquid / methods*, statistics & numerical data Corpus Striatum / metabolism Humans Hydroxylation Loxapine / analogs & derivatives, blood* Rats Spiperone / metabolism |
| Chemical | |
Reg. No./Substance:
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14028-44-5/Amoxapine; 1977-10-2/Loxapine; 61443-77-4/8-hydroxyloxapine; 749-02-0/Spiperone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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