Document Detail


Simultaneous quantification of total eicosapentaenoic acid, docosahexaenoic acid and arachidonic acid in plasma by high-performance liquid chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  20737653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the simultaneous quantification of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) in human plasma by HPLC-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. Free and esterified forms of fatty acids were hydrolysed from plasma samples in the presence of an internal standard and subjected to liquid-liquid extraction. The chromatographic run time was 3.5 min per sample. The assay was linear from 0.5 to 300 mg/L (r(2) > 0.997, n = 18). Based on matrix addition, accuracy deviation was <15%, except for AA at 10 mg/L (30-90%), whereas precision was <8% for all fatty acids studied. The method was applied to the measurement of these omega-3 fatty acids in a fish oil supplement study with healthy volunteers. Healthy males (n = 4) were administered a supplement containing 465 mg EPA and 375 mg DHA per capsule (Omacor®). A dose of two capsules was given daily over a 4 week period. Pre-treatment concentrations varied between subjects for EPA (17-68 mg/L), DHA (36-63 mg/L) and AA (121-248 mg/L). During the dosing period EPA increased 460-480% from the baseline concentration, while DHA increased 150-160%. The EPA-AA ratio increased from 0.07-0.56 to 0.3-3.1 after 4 weeks of dosing. In conclusion, the method described could be suitable for monitoring EPA, DHA and AA in clinical studies that may aid in achieving optimal concentrations of these fatty acids in patients who could be at risk of sudden cardiac death.
Authors:
Paul Salm; Paul J Taylor; Karam Kostner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-25
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  25     ISSN:  1099-0801     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-10     Completed Date:  2011-08-26     Revised Date:  2011-09-16    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  652-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Affiliation:
Australian Bioanalytical Services Pty Ltd, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia. ausbio@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Arachidonic Acid / blood*,  pharmacokinetics
Chromatography, High Pressure Liquid / methods*
Dietary Supplements
Docosahexaenoic Acids / administration & dosage,  blood*,  pharmacokinetics
Drug Combinations
Eicosapentaenoic Acid / administration & dosage,  blood*,  pharmacokinetics
Fish Oils / administration & dosage
Humans
Linear Models
Male
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Chemical
Reg. No./Substance:
0/Drug Combinations; 0/Fish Oils; 0/Omacor; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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