| Simultaneous quantification of rosiglitazone and its two major metabolites, N-desmethyl and p-hydroxy rosiglitazone in human plasma by liquid chromatography/tandem mass spectrometry: application to a pharmacokinetic study. | |
| | |
MedLine Citation:
|
PMID: 19477697 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We present a simple, rapid, and sensitive liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method for the simultaneous quantification of rosiglitazone and its two major metabolites via CYP2C8/9, N-desmethyl and p-hydroxy rosiglitazone, in human plasma. The procedure was developed and validated using rosiglitazone-d(3) as the internal standard. Plasma samples (0.1 ml) were prepared using a simple deproteinization procedure with 0.2 ml of acetonitrile containing 40 ng/ml of rosiglitazone-d(3). Chromatographic separation was carried out on a Luna C18 column (100 mm x 2.0 mm, 3-microm particle size) using an isocratic mobile phase consisting of a 60:40 (v/v) mixture of acetonitrile and 0.1% formic acid((aq)). Each sample was run at 0.2 ml/min for a total run time of 2.5 min per sample. Detection and quantification were performed using a mass spectrometer in selected reaction-monitoring mode with positive electrospray ionization at m/z 358.1-->135.1 for rosiglitazone, m/z 344.2-->121.1 for N-desmethyl rosiglitazone, m/z 374.1-->151.1 for p-hydroxy rosiglitazone, and m/z 361.1-->138.1 for rosiglitazone-d(3). The linear ranges of concentration for rosiglitazone, N-desmethyl rosiglitazone, and p-hydroxy rosiglitazone were 1-500, 1-150, and 1-25 ng/ml, respectively, with a lower limit of quantification of 1 ng/ml for all analytes. The coefficient of variation for assay precision was less than 14.4%, and the accuracy was 93.3-112.3%. No relevant cross-talk and matrix effect were observed. This method was successfully applied to a pharmacokinetic study after oral administration of a 4-mg rosiglitazone tablet to healthy male Korean volunteers. |
| | |
Authors:
|
Kwon-Bok Kim; Dong Jun Lee; Chang-Woo Yeo; Jae-Gook Shin; Soo Kyung Bae |
Related Documents
:
|
17080507 - A rapid and specific approach for direct measurement of pravastatin concentration in pl... 10746957 - 'high throughput' solid-phase extraction technology and turbo ionspray lc-ms-ms applied... 16709467 - First lc-ms/ms electrospray ionization validated method for the quantification of perin... 20482447 - A less sensitive detector does not necessarily result in a less sensitive method: fast ... 19652507 - Erythrocyte methotrexate-polyglutamate assay using fluorescence polarization immunoassa... 14648607 - A sensitive and selective determination method of histamine by hplc with intramolecular... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2009-05-13 |
Journal Detail:
|
Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 877 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-06-15 Completed Date: 2009-08-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1951-6 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, South Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromatography, Liquid
/
methods* Humans Hypoglycemic Agents / blood*, chemistry, pharmacokinetics Male Tandem Mass Spectrometry / methods* Thiazolidinediones / blood*, chemistry, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
|
0/Hypoglycemic Agents; 0/Thiazolidinediones; 122320-73-4/rosiglitazone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Capillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes.
Next Document: Kava hepatotoxicity: Regulatory data selection and causality assessment.