| Simultaneous production and characterization of medium-chain-length polyhydroxyalkanoates and alginate oligosaccharides by Pseudomonas mendocina NK-01. | |
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MedLine Citation:
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PMID: 21617928 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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When Pseudomonas mendocina NK-01 was cultivated in a 200-L fermentor using glucose as carbon source, 0.316 g L(-1) medium-chain-length polyhydroxyalkanoate (PHA(MCL)) and 0.57 g L(-1) alginate oligosaccharides (AO) were obtained at the end of the process. GC/MS was used to characterize the PHA(MCL), which was found to be a polymer mainly consisting of 3HO (3-hydroxyoctanoate) and 3HD (3-hydroxydecanoate). T (m) and T (g) values for the PHA(MCL) were 51.03°C and -41.21°C, respectively, by DSC. Its decomposition temperature was about 300°C. The elongation at break was 700% under 12 MPa stress. MS and GPC were also carried out to characterize the AO which had weight-average molecular weights of 1,546 and 1,029 Da, respectively, for the two main components at the end of the fermentation process. MS analysis revealed that the AO were consisted of β-D: -mannuronic acid and/or α-L: -guluronic acid, and the β-D: -mannuronic acid and/or α-L: -guluronic acid residues were partially acetylated at position C2 or C3. |
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Authors:
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Wenbin Guo; Cunjiang Song; Meimei Kong; Weitao Geng; Yuanyuan Wang; Shufang Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-27 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: - ISSN: 1432-0614 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Key Laboratory of Molecular MicroBiology and Technology, Ministry of Education, Department of MicroBiology, College of Life Sciences, Nankai University, Tianjin, 300071, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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