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Simultaneous production and characterization of medium-chain-length polyhydroxyalkanoates and alginate oligosaccharides by Pseudomonas mendocina NK-01.
MedLine Citation:
PMID:  21617928     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
When Pseudomonas mendocina NK-01 was cultivated in a 200-L fermentor using glucose as carbon source, 0.316 g L(-1) medium-chain-length polyhydroxyalkanoate (PHA(MCL)) and 0.57 g L(-1) alginate oligosaccharides (AO) were obtained at the end of the process. GC/MS was used to characterize the PHA(MCL), which was found to be a polymer mainly consisting of 3HO (3-hydroxyoctanoate) and 3HD (3-hydroxydecanoate). T (m) and T (g) values for the PHA(MCL) were 51.03°C and -41.21°C, respectively, by DSC. Its decomposition temperature was about 300°C. The elongation at break was 700% under 12 MPa stress. MS and GPC were also carried out to characterize the AO which had weight-average molecular weights of 1,546 and 1,029 Da, respectively, for the two main components at the end of the fermentation process. MS analysis revealed that the AO were consisted of β-D: -mannuronic acid and/or α-L: -guluronic acid, and the β-D: -mannuronic acid and/or α-L: -guluronic acid residues were partially acetylated at position C2 or C3.
Authors:
Wenbin Guo; Cunjiang Song; Meimei Kong; Weitao Geng; Yuanyuan Wang; Shufang Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-27
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Molecular MicroBiology and Technology, Ministry of Education, Department of MicroBiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
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