Document Detail


Simultaneous production of catalase, glucose oxidase and gluconic acid by Aspergillus niger mutant.
MedLine Citation:
PMID:  10333558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The production of gluconic acid, extracellular glucose oxidase and catalase in submerged culture by a number of biochemical mutants has been evaluated. Optimization of stirrer speed, time cultivation and buffering action of some chemicals on glucose oxidase, catalase and gluconic acid production by the most active mutant, AM-11, grown in a 3-L glass bioreactor was investigated. Three hundred rpm appeared to be optimum to ensure good growth and best glucose oxidase production, but gluconic acid or catalase activity obtained maximal value at 500 or 900 rpm, respectively. Significant increase of dissolved oxygen concentration in culture (16-21%) and extracellular catalase activity were obtained when the traditional aeration was employed together with automatic dosed hydrogen peroxide.
Authors:
J Fiedurek; A Gromada; J Pielecki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta microbiologica Polonica     Volume:  47     ISSN:  0137-1320     ISO Abbreviation:  Acta Microbiol. Pol.     Publication Date:  1998  
Date Detail:
Created Date:  1999-06-17     Completed Date:  1999-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7610362     Medline TA:  Acta Microbiol Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  355-64     Citation Subset:  IM    
Affiliation:
Department of Industrial Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus niger / genetics,  growth & development,  metabolism*
Bioreactors
Catalase / biosynthesis*
Culture Media
Glass
Gluconates / metabolism*
Glucose / metabolism
Glucose Oxidase / biosynthesis*
Hydrogen Peroxide / metabolism
Mutation
Oxygen / pharmacology
Silicates
Sodium Hydroxide / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Gluconates; 0/Silicates; 1310-73-2/Sodium Hydroxide; 1332-09-8/pumice; 50-99-7/Glucose; 526-95-4/gluconic acid; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; EC 1.1.3.4/Glucose Oxidase; EC 1.11.1.6/Catalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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