Document Detail


Simultaneous production of R-bands and either replication patterns or sister chromatid differentiation.
MedLine Citation:
PMID:  2416669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dye triplet chromomycin/methyl green/DAPI and some related dye combinations were applied to bromodeoxyuridine (BrdU)-substituted and non-substituted chromosomes. In both kinds of chromosome preparations, tri-staining followed by observation at pH 7 resulted in well defined R-bands (excitation wavelength 436 nm) and a mixed DA-DAPI/Q-banding pattern (360 nm). Two approaches have given satisfactory expression both of reverse bands and of the differential BrdU-substitution present in metaphase chromosomes: (1) direct tri-staining at pH 7 and mounting of the preparations at pH 11; or (2) pretreatment of the preparations with Hoechst 33258 plus UV and hot buffer followed by tri-staining and mounting at pH 7. These methods should prove useful for routine chromosome analysis and, in combination with BrdU-labelling, in studies of chromosome structure and replication.
Authors:
P M Kroisel; W Rosenkranz; D Schweizer
Related Documents :
3880379 - A direct technique for the preparation of chromosomes from early equine embryos.
12163909 - Evidence of spotted fever group rickettsiae in state of rio de janeiro, brazil.
2272499 - The pattern of x-chromosome inactivation in the embryonic and extra-embryonic tissues o...
48449 - Hoechst 33258 fluorescent staining of drosophila chromosomes.
87099 - An analysis of factors for the differential staining of sister chromatids in human chro...
598839 - Inhibition of the development of q-bands on human chromosomes by netropsin.
22010049 - Loci affecting gamma-glutamyl transferase in adults and adolescents show age × snp inte...
8223499 - Update on target theory as applied to chromosomal aberrations.
1642939 - Gastroduodenal dysfunction as a cause of gastric bacterial overgrowth in patients under...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  71     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1985  
Date Detail:
Created Date:  1986-01-29     Completed Date:  1986-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  333-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bromodeoxyuridine / pharmacology
Chromosome Banding / methods*
Humans
Karyotyping
Lymphocytes / ultrastructure
Sister Chromatid Exchange*
Staining and Labeling
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates inter...
Next Document:  [Serologic cross reactions of group B streptococci].