Document Detail

Simultaneous production of R-bands and either replication patterns or sister chromatid differentiation.
MedLine Citation:
PMID:  2416669     Owner:  NLM     Status:  MEDLINE    
The dye triplet chromomycin/methyl green/DAPI and some related dye combinations were applied to bromodeoxyuridine (BrdU)-substituted and non-substituted chromosomes. In both kinds of chromosome preparations, tri-staining followed by observation at pH 7 resulted in well defined R-bands (excitation wavelength 436 nm) and a mixed DA-DAPI/Q-banding pattern (360 nm). Two approaches have given satisfactory expression both of reverse bands and of the differential BrdU-substitution present in metaphase chromosomes: (1) direct tri-staining at pH 7 and mounting of the preparations at pH 11; or (2) pretreatment of the preparations with Hoechst 33258 plus UV and hot buffer followed by tri-staining and mounting at pH 7. These methods should prove useful for routine chromosome analysis and, in combination with BrdU-labelling, in studies of chromosome structure and replication.
P M Kroisel; W Rosenkranz; D Schweizer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  71     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1985  
Date Detail:
Created Date:  1986-01-29     Completed Date:  1986-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  333-41     Citation Subset:  IM    
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MeSH Terms
Bromodeoxyuridine / pharmacology
Chromosome Banding / methods*
Lymphocytes / ultrastructure
Sister Chromatid Exchange*
Staining and Labeling
Reg. No./Substance:

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