Document Detail

Simultaneous optical measurements of cell motility and growth.
MedLine Citation:
PMID:  22025987     Owner:  NLM     Status:  PubMed-not-MEDLINE    
It has recently been shown that spatial light interference microscopy (SLIM) developed in our laboratory can be used to quantify the dry mass growth of single cells with femtogram sensitivity [M. Mir et al., Proc. Nat. Acad. Sci. 108, 32 (2011)]. Here we show that it is possible to measure the motility of single cells in conjunction with the dry mass measurements. Specifically the effect of poly-L-lysine substrate on the dry mass growth of Drosophila S2 cells is studied. By measuring the mean square displacement of single cells and clusters it is shown that cells that adhere better to the surface are unable to grow. Using such a technique it is possible to measure both growth and morphogenesis, two of the cornerstones of developmental biology.
Shamira Sridharan; Mustafa Mir; Gabriel Popescu
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Publication Detail:
Type:  Journal Article     Date:  2011-09-22
Journal Detail:
Title:  Biomedical optics express     Volume:  2     ISSN:  2156-7085     ISO Abbreviation:  Biomed Opt Express     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-25     Completed Date:  2011-11-10     Revised Date:  2014-10-30    
Medline Journal Info:
Nlm Unique ID:  101540630     Medline TA:  Biomed Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2815-20     Citation Subset:  -    
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