Document Detail

Simultaneous noninvasive clinical measurement of lens autofluorescence and rayleigh scattering using a fluorescence biomicroscope.
MedLine Citation:
PMID:  23294769     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Lens autofluorescence increases with the age of the subject, and the fluorophores responsible are associated with cataract, retinopathy, and other complications of diabetes. We built a scanning confocal lens fluorescence biomicroscope suitable for routine clinical measurement of lens autofluorescence and light scattering and report data from 127 healthy subjects.
METHOD: The fluorescence biomicroscope focuses a beam of light from a blue light-emitting diode on the lens and measures fluorescent green light and blue scattered light using a sensitive silicon photomultiplier. The system includes a target fixation light and a video camera for alignment and automatic pupil tracking. Under software control, a volume of measurement is scanned from behind the posterior lens capsule, through the lens to the aqueous humor, and then back again. Software computes the average ratio of lens autofluorescence to scattered light in the central portion of the lens. Self-reported healthy nondiabetic subjects were examined by an optometrist; if their eyes were healthy and without significant cataract, they were entered into the study.
RESULTS: Valid lens autofluorescence data were collected from 127 subjects between 21 and 70 years of age. A linear model for lens autofluorescence intensity with age was highly statistically significant, and the improvement in fit for higher-order polynomial models was not statistically significant. The ratio of lens autofluorescence to light scatter was also calculated; regression analysis showed significant curvature for the relationship of the fluorescence ratio to age, so a nonlinear model was used to estimate the mean ratio of autofluorescence to scatter and its prediction intervals as a function of age.
CONCLUSIONS: Our observation of a strongly significant linear regression of fluorescence intensity with age of the subjects agrees with the results from previous studies, as does a nonlinear model for the fluorescence ratio. The fluorescence biomicroscope enables the clinician to identify patients with fluorescence ratio significantly higher than expected for their age.
John Burd; Stephen Lum; Frederick Cahn; Keith Ignotz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-01
Journal Detail:
Title:  Journal of diabetes science and technology     Volume:  6     ISSN:  1932-2968     ISO Abbreviation:  J Diabetes Sci Technol     Publication Date:  2012  
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-08-28     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  101306166     Medline TA:  J Diabetes Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1251-9     Citation Subset:  IM    
Copyright Information:
© 2012 Diabetes Technology Society.
Freedom Meditech, San Diego, CA, USA.
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MeSH Terms
Age Factors
Glycosylation End Products, Advanced / analysis*
Lens, Crystalline / pathology*
Microscopy, Confocal / instrumentation,  methods*
Middle Aged
Optical Imaging / instrumentation,  methods*
Young Adult
Reg. No./Substance:
0/Glycosylation End Products, Advanced

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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