Document Detail

Simultaneous monitoring of organic acids and sugars in fresh and processed apple juice by Fourier transform infrared-attenuated total reflection spectroscopy.
MedLine Citation:
PMID:  14686782     Owner:  NLM     Status:  MEDLINE    
A combination of Fourier transform infrared spectroscopy (FT-IR) and chemometrics was used as a screening tool for the determination of sugars and organic acids such as sucrose, glucose, fructose, sorbitol, citric acid, and malic acid in processed commercial and extracted fresh apple juices. Prepared samples of synthetic apple juice in different constituent concentration ranges were scanned by attenuated total reflectance (ATR) accessory and the spectral region in the range between 950 and 1500 cm(-1) was selected for calibration model development using partial least squares (PLS) regression and principal component regression (PCR). The calibration models were successfully validated by high-performance liquid chromatography (HPLC) measurements against several commercial juice varieties as well as juice extracted from different apple varieties to provide an overall R2 correlation of 0.998. The present study demonstrates that Fourier transform infrared spectroscopy could be used for rapid and nondestructive determination of multiple constituents in commercial and fresh apple juices. Results indicate this approach to be a rapid and cost-effective tool for routine monitoring of multiple constituents in a fruit juice production facility.
Joseph Irudayaraj; Jagdish Tewari
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied spectroscopy     Volume:  57     ISSN:  0003-7028     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-22     Completed Date:  2004-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1599-604     Citation Subset:  IM    
Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
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MeSH Terms
Beverages / analysis*
Carbohydrates / analysis*
Chromatography, High Pressure Liquid
Fruit / chemistry*
Malus / chemistry*
Organic Chemicals / analysis*
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared / methods*
Reg. No./Substance:
0/Carbohydrates; 0/Organic Chemicals

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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