Document Detail


Simultaneous measurement of rat brain cortex PtO2 using EPR oximetry and a fluorescence fiber-optic sensor during normoxia and hyperoxia.
MedLine Citation:
PMID:  15798296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electron paramagnetic resonance (EPR) oximetry is a promising, relatively non-invasive method of monitoring tissue partial pressure of oxygen (PtO(2)) that has proven useful in following changes in PtO(2) under various physiologic and pathophysiologic conditions. Optimal utilization of the method will be facilitated by systematic comparisons with other available methods. Here, we report on the absolute values and changes of rat brain PtO(2) using EPR oximetry and the OxyLite, an oxygen monitor based on fluorescence quenching, at adjacent locations in the same brain. EPR oximetry utilizes an implanted oxygen-sensitive material and reports tissue PtO(2) at the surface of the material. OxyLite measures PtO(2) using the fluorescence lifetime of a chromophore fixed to the tip of an optical fiber that is inserted into tissue. Measurements were made at a depth of 2-3 mm into the cortex during normoxia and during breathing of carbogen (95% O(2):5% CO(2)) followed by a return to normoxia. We conclude that in this study (1) PtO(2) values reported by the two methods are similar but not exactly the same, (2) both methods can record a baseline and rapid changes in PtO(2), (3) changes in PtO(2) induced by increasing FiO(2) from 0.26 to 0.95 (carbogen) were similar by the two methods and (4) in some rats breathing carbogen, absolute values of PtO(2) were above the sensitive range of the OxyLite method.
Authors:
Julia A O'Hara; Huagang Hou; Eugene Demidenko; Roger J Springett; Nadeem Khan; Harold M Swartz
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-02-25
Journal Detail:
Title:  Physiological measurement     Volume:  26     ISSN:  0967-3334     ISO Abbreviation:  Physiol Meas     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-03-30     Completed Date:  2005-08-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9306921     Medline TA:  Physiol Meas     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-13     Citation Subset:  IM    
Affiliation:
EPR Center for the Study of Viable Systems, Dartmouth Medical School, Hanover, NH 03755, USA. julia.ohara@dartmouth.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Electron Spin Resonance Spectroscopy / methods*
Fiber Optic Technology / instrumentation
Hyperoxia / metabolism*
Optical Fibers
Oximetry / methods*
Oxygen / metabolism*
Rats
Rats, Inbred F344
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence / instrumentation,  methods*
Transducers
Grant Support
ID/Acronym/Agency:
P01 EB002180/EB/NIBIB NIH HHS; P41 EB002032/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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