Document Detail

Simultaneous measurement of phosphatidylglycerol and disaturated-phosphatidylcholine palmitate kinetics from alveolar surfactant. Study in infants with stable isotope tracer, coupled with isotope ratio mass spectrometry.
MedLine Citation:
PMID:  22012664     Owner:  NLM     Status:  In-Data-Review    
Disaturated-phosphatidylcholine (DSPC) and phosphatidylglycerol (PG) are respectively the first and the third most abundant phospholipid in human alveolar surfactant. Their concentration decreases in airway surfactant of adults and infants with respiratory distress syndrome and cystic fibrosis. In this study, we used mass spectrometry (IRMS) to investigate the turnover of DSPC and PG in tracheal aspirates (TA) obtained from infants with normal or diseased lungs. We studied eight infants requiring mechanical ventilation: two with no lung disease, four with diaphragmatic hernia, one with ATP-binding cassette sub-family A member 3 heterozygote mutation and one with sepsis. Patients received deuterated water for 48 h as metabolic precursors of palmitate-DSPC and palmitate-PG. Serial TAs were obtained every 6 h for five days or until extubation. DSPC and PG were isolated from TA by column and high-performance thin layer chromatography. Deuterium enrichments of palmitate-DSPC and PG residues were measured by IRMS coupled with a gas chromatographer. Median secretion time (ST), peak time (PT) and fractional synthesis rate (FSR) were 3.7 [0.9- 13.4] h, 71.0 [52.2 - 85.2] h and 6.6 [6.3 - 11.1] %/day for DSPC and 19.3 [6.4 - 22.8] h, 49.0 [33.0 - 52.5] h and 5.8 [4.8 - 10.9] %/day for PG. This study shows that it is feasible to use deuterium derived from body water to trace simultaneously airway surfactant DSPC and PG in humans. When compared within the same patient, DSPC and PG had similar fractional synthesis rates, but PG had a shorter PT, suggesting differences in the life cycle of these essential surfactant components. Copyright © 2011 John Wiley & Sons, Ltd.
Luca Vedovelli; Aldo Baritussio; Virgilio Paolo Carnielli; Manuela Simonato; Pietro Giusti; Paola Elisa Cogo
Related Documents :
17243184 - Palivizumab efficacy in preterm infants with gestational age < or = 30 weeks without br...
2277284 - Echocardiographic correlates of survival in severe bronchopulmonary dysplasia.
17426644 - Retinoids increase lung elastin expression but fail to alter morphology or angiogenesis...
19574444 - Circulating endothelial progenitor cells in preterm infants with bronchopulmonary dyspl...
22161404 - Early additional food and fluids for healthy breastfed full-term infants.
8233254 - An ethical justification for emergency, coerced cesarean delivery.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  46     ISSN:  1096-9888     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  986-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Department of Anesthesiology and Pharmacology, University of Padua, Padua, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Isotope-coded dimethyl tagging for differential quantification of posttranslational protein carbonyl...
Next Document:  Determination of the glycation sites of Bacillus anthracis neoglycoconjugate vaccine by MALDI-TOF/TO...