Document Detail


Simultaneous measurement of the maximum oscillation amplitude and the transient decay time constant of the QCM reveals stiffness changes of the adlayer.
MedLine Citation:
PMID:  14504678     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interpretation of adsorption kinetics measured with a quartz crystal microbalance (QCM) can be difficult for adlayers undergoing modification of their mechanical properties. We have studied the behavior of the oscillation amplitude, A(0), and the decay time constant, tau, of quartz during adsorption of proteins and cells, by use of a home-made QCM. We are able to measure simultaneously the frequency, f, the dissipation factor, D, the maximum amplitude, A(0), and the transient decay time constant, tau, every 300 ms in liquid, gaseous, or vacuum environments. This analysis enables adsorption and modification of liquid/mass properties to be distinguished. Moreover the surface coverage and the stiffness of the adlayer can be estimated. These improvements promise to increase the appeal of QCM methodology for any applications measuring intimate contact of a dynamic material with a solid surface.
Authors:
C Galli Marxer; M Collaud Coen; H Bissig; U F Greber; L Schlapbach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-07-09
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  377     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-23     Completed Date:  2003-11-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  570-7     Citation Subset:  IM    
Affiliation:
Solid State Physics Research Group, University of Fribourg, Pérolles, 1700, Fribourg, Switzerland. Carine.Galli@unifr.ch
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Biosensing Techniques* / instrumentation,  methods
Cell Adhesion / physiology
Chemistry, Physical
Elasticity
Electrodes
Epithelial Cells
Fibronectins / analysis
Kinetics
Models, Chemical
Physicochemical Phenomena
Proteins / chemistry*
Quartz*
Staphylococcal Protein A / analysis
Tumor Cells, Cultured
Viscosity
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Proteins; 0/Staphylococcal Protein A; 14808-60-7/Quartz

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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