Document Detail

Simultaneous measurement of fluoroquinolones in eggs by a combination of supercritical fluid extraction and high pressure liquid chromatography.
MedLine Citation:
PMID:  12843663     Owner:  NLM     Status:  MEDLINE    
Simultaneous detection of the fluoroquinolone antibiotics ciprofloxacin, enrofloxacin, ofloxacin, and norfloxacin in eggs by a combination of supercritical fluid extraction (SFE) and high pressure liquid chromatography (HPLC) was studied. Lipid matrices that have been considered to result in poor extraction and isolation of fluoroquinolones in eggs were removed first by SFE with supercritical CO(2) alone, and then the fluoroquinolones were extracted by SFE with supercritical CO(2) containing 20% (v/v) methanol for HPLC analysis. A time-course study of the extraction of lipid matrices of eggs suggested that the SFE method successfully removed the matrices within 20 min. When the fluoroquinolones added to control eggs were extracted by SFE, the extraction efficiency was similar to that by the solvent extraction method, giving the recovery percentages from 83 to 96% in a 40 min-extraction time. The fluoroquinolones extracted from eggs by SFE were analyzed simultaneously by HPLC equipped with a fluorescence detector with detection sensitivity at about 10 ppb for the detection limit. The standard calibration profiles of fluoroquinolones showed linear responses to HPLC, showing more than 0.995 for the mean r(2) value. This is the first report of the simultaneous measurement of fluoroquinolones in eggs by a combination of SFE and HPLC. Using the SFE method allowed us to avoid extensive sample preparation such as solvent extraction and chromatographic cleanup that are basically required in extraction of fluoroquinolones.
Jae Han Shim; Mi Hyun Lee; Mi Ra Kim; Chang Joo Lee; In Seon Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  67     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-04     Completed Date:  2004-04-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1342-8     Citation Subset:  IM    
Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, South Korea.
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MeSH Terms
Anti-Bacterial Agents / analysis*
Chromatography, High Pressure Liquid / instrumentation,  methods*
Chromatography, Supercritical Fluid / methods*,  standards
Ciprofloxacin / analysis,  isolation & purification
Eggs / analysis*
Fluoroquinolones / analysis*,  isolation & purification
Food Contamination / analysis
Norfloxacin / analysis,  isolation & purification
Ofloxacin / analysis,  isolation & purification
Quinolones / analysis,  isolation & purification
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fluoroquinolones; 0/Quinolones; 0/Solvents; 70458-96-7/Norfloxacin; 82419-36-1/Ofloxacin; 85721-33-1/Ciprofloxacin; 93106-60-6/enrofloxacin

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