| Simultaneous liquid-chromatographic analysis for 4-hydroxy-3-methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine. | |
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MedLine Citation:
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PMID: 7353279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0.7 cm column). The two analytes are eluted from the column with 10 mL of 3 mol/L sodium chloride, and 2 microL of the eluate, injected directly into the liquid chromatograph, is chromatographed at 40 degrees C. This column contains mu Bondapak C18, and the mobile phase is 100 mmol/L potassium phosphate buffer, pH 6.7, containing 0.5 mmol of tetrabutylammonium phosphate per liter. Detector sensitivity is 50 nA full scale. Analytical recoveries of I and II are 80--84% and 48--49%, respectively; the coefficients of variation for various concentrations in urine are about 10%. |
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Authors:
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S J Soldin; J G Hill |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 26 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1980 Feb |
Date Detail:
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Created Date: 1980-04-17 Completed Date: 1980-04-17 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 291-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Gland Neoplasms
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urine Chromatography, High Pressure Liquid / methods* Homovanillic Acid / urine* Humans Neuroblastoma / urine Phenylacetates / urine* Pheochromocytoma / urine Vanilmandelic Acid / urine* |
| Chemical | |
Reg. No./Substance:
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0/Phenylacetates; 306-08-1/Homovanillic Acid; 55-10-7/Vanilmandelic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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