Document Detail

Simultaneous exposure of Xenopus A6 kidney epithelial cells to concurrent mild sodium arsenite and heat stress results in enhanced hsp30 and hsp70 gene expression and the acquisition of thermotolerance.
MedLine Citation:
PMID:  19358893     Owner:  NLM     Status:  MEDLINE    
In this study, we examined the effect of concurrent low concentrations of sodium arsenite and mild heat shock temperatures on hsp30 and hsp70 gene expression in Xenopus A6 kidney epithelial cells. RNA blot hybridization and immunoblot analysis revealed that exposure of A6 cells to 1-10 microM sodium arsenite at a mild heat shock temperature of 30 degrees C enhanced hsp30 and hsp70 gene expression to a much greater extent than found with either stress individually. In cells treated simultaneously with 10 microM sodium arsenite and different heat shock temperatures, enhanced accumulation of HSP30 and HSP70 protein was first detected at 26 degrees C with larger responses at 28 and 30 degrees C. HSF1 activity was involved in combined stress-induced hsp gene expression since the HSF1 activation inhibitor, KNK437, inhibited HSP30 and HSP70 accumulation. Immunocytochemical analysis revealed that HSP30 was present in a granular pattern primarily in the cytoplasm in cells treated simultaneously with both stresses. Finally, prior exposure of A6 cells to concurrent sodium arsenite (10 microM) and heat shock (30 degrees C) treatment conferred thermotolerance since it protected them against a subsequent thermal challenge (37 degrees C). Acquired thermotolerance was not observed with cells treated with the two mild stresses individually.
Jordan T F Young; Julie Gauley; John J Heikkila
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-07
Journal Detail:
Title:  Comparative biochemistry and physiology. Part A, Molecular & integrative physiology     Volume:  153     ISSN:  1531-4332     ISO Abbreviation:  Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806096     Medline TA:  Comp Biochem Physiol A Mol Integr Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  417-24     Citation Subset:  IM    
Department of Biology, University of Waterloo, Waterloo, ON, Canada N2L 3G1.
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MeSH Terms
Adaptation, Physiological*
Arsenites / pharmacology*
Blotting, Northern
Epithelial Cells / drug effects,  metabolism
HSP30 Heat-Shock Proteins / genetics*
HSP70 Heat-Shock Proteins / genetics*
Hot Temperature*
Kidney / cytology,  drug effects*,  metabolism,  physiology
Microscopy, Confocal
Sodium Compounds / pharmacology*
Reg. No./Substance:
0/Arsenites; 0/HSP30 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/Sodium Compounds; 13768-07-5/sodium arsenite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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