Document Detail


Simultaneous determination of water-soluble vitamins in SRM 1849 Infant/Adult Nutritional Formula powder by liquid chromatography-isotope dilution mass spectrometry.
MedLine Citation:
PMID:  20063152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Assessing dietary intake of vitamins from all sources, including foods, dietary supplements, and fortified foods, would be aided considerably by having analytical methodologies that are capable of simultaneous determination of several vitamins. Vitamins naturally present in foods may occur in different chemical forms, with levels ranging over several orders of magnitude. Vitamins in dietary supplements and fortified foods, however, are typically added in a single chemical form, and matrix issues are usually not as complex. These sources should thus be relatively amenable to approaches that aim for simultaneous determination of multiple vitamins. Our recent work has focused on development of liquid chromatography (LC)-UV/fluorescence and LC-tandem mass spectrometry methods for the simultaneous determination of water-soluble vitamins (thiamine, niacin, pyridoxine, pantothenic acid, folic acid, biotin, and riboflavin) in dietary supplement tablets and fortified foods, such as formula powders and breakfast cereals. As part of the validation of our methods and collaboration in characterization of a new NIST SRM 1849 Infant/Adult Nutritional Formula powder, we report data on SRM 1849 using isotope dilution mass spectrometric methods. Use of available NIST Standard Reference Materials(R) as test matrices in our method development and validation gives a benchmark for future application of these methods. We compare three chromatographic approaches and provide data on stability of vitamin standard solutions for LC-based multiple vitamin determinations.
Authors:
Robert J Goldschmidt; Wayne R Wolf
Publication Detail:
Type:  Journal Article; Validation Studies     Date:  2010-01-10
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  397     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  471-81     Citation Subset:  IM    
Affiliation:
Food Composition Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chromatography, Liquid / methods*
Food, Formulated / analysis*
Humans
Indicator Dilution Techniques
Infant
Isotopes / analysis
Mass Spectrometry / methods*
Reference Standards
Vitamins / analysis*
Chemical
Reg. No./Substance:
0/Isotopes; 0/Vitamins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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