Document Detail

Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection.
MedLine Citation:
PMID:  15171934     Owner:  NLM     Status:  MEDLINE    
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIFD) coupled to in vivo microdialysis sampling was used in order to monitor simultaneously a drug and several neurotransmitters in the brain extracellular fluid. Determination of the antiepileptic drug vigabatrin and the amino acid neurotransmitters glutamate (Glu), l-aspartate (l-Asp) and gamma-aminobutyric acid (GABA) was performed on low-concentration samples which were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) and separated using a pH 9.2 75 mM sodium borate running buffer containing 60 mM sodium dodecyl sulfate (SDS) and 5mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD). Glu, l-Asp and vigabatrin derivatized at a concentration of 1.0 x 10(-9) M, and GABA derivatized at a concentration of 5.0 x 10(-9) M, produced peaks with signal-to-noise ratios of 8:1, 8:1, 4:1 and 5:1, respectively. The nature of the neurotransmitter peaks found in rat brain microdialysates was confirmed by both electrophoretic and pharmacological validations. This method was used for monitoring vigabatrin and amino acid neurotransmitters in microdialysates from the rat striatum during intracerebral infusion of the drug and revealed rapid vigabatrin-induced changes in GABA and Glu levels. This original application of CE-LIFD coupled to microdialysis represents a powerful tool for pharmacokinetic/pharmacodynamic investigations.
Nadia Benturquia; Sandrine Parrot; Valérie Sauvinet; Bernard Renaud; Luc Denoroy
Related Documents :
3507224 - Micro-scale sequence analysis from the n-terminus of peptides using the fluorogenic edm...
19691964 - Microchip electrophoresis with chemiluminescence detection for assaying ascorbic acid a...
6735684 - Hydroxylysine glycosides: preparation and analysis by reverse phase high performance li...
12831204 - Application of a new chiral derivatizing agent to the enantioseparation of secondary am...
8239994 - Pathways of trans,trans-muconaldehyde metabolism in mouse liver cytosol: reversibility ...
1545354 - Ester and amide prodrugs of ibuprofen and naproxen: synthesis, anti-inflammatory activi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  806     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-02     Completed Date:  2005-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
Laboratoire de Neuropharmacologie et Neurochimie, INSERM U512, Faculté de Pharmacie, et Institut Fédératif des Neurosciences de Lyon, IFR 19, Université Claude Bernard, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis*
Corpus Striatum / chemistry*
Electrophoresis, Capillary / methods*
Neurotransmitter Agents / analysis*
Rats, Sprague-Dawley
Spectrometry, Fluorescence / methods*
Vigabatrin / analysis*
Reg. No./Substance:
0/Amino Acids; 0/Neurotransmitter Agents; 60643-86-9/Vigabatrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Interval immobilization technique for recognition toward a highly hydrophilic cyanobacterium toxin.
Next Document:  Liquid chromatography-mass spectrometry analysis of hydroxylated polycyclic aromatic hydrocarbons, f...