Document Detail


Simultaneous determination of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acids in human urine by thin layer chromatography.
MedLine Citation:
PMID:  8792867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.
Authors:
G Alemany; A Gamundí; C Rosselló; R Rial
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  10     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:    1996 May-Jun
Date Detail:
Created Date:  1996-11-13     Completed Date:  1996-11-13     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  144-5     Citation Subset:  IM    
Affiliation:
Dept. de Biologia i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Thin Layer*
Homovanillic Acid / urine*
Hydroxyindoleacetic Acid / urine*
Molybdenum
Phenylpropionates / urine
Reference Standards
Reproducibility of Results
Tungsten Compounds
Vanilmandelic Acid / urine*
Chemical
Reg. No./Substance:
0/Folin's phenol reagent; 0/Phenylpropionates; 0/Tungsten Compounds; 306-08-1/Homovanillic Acid; 306-23-0/4-hydroxyphenyllactic acid; 54-16-0/Hydroxyindoleacetic Acid; 55-10-7/Vanilmandelic Acid; 7439-98-7/Molybdenum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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