Document Detail


Simultaneous determination of uronates found in polysaccharides from natural products by HPLC with fluorometric detection.
MedLine Citation:
PMID:  22824507     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A sensitive high-performance liquid chromatographic (HPLC) method for the determination of uronates isolated from polysaccharides found in natural products such as glycosaminoglycans and alginate is described. Preparation of iduronate, guluronate, and mannuronate as analytical standards for high performance liquid chromatography was achieved by depolymerization of dermatan sulfate and alginate in 2.5molL(-1) trifluoroacetic acid at 100°C for 6h. Structures of resulting products (iduronate, guluronate, and mannuronate) were characterized by 600MHz (1)H NMR. Five uronates (glucuronate, iduronate, mannuronate, guluronate, and galacturonate) were separated on a Dionex CarboPac PA1 column using an isocratic elution with 8mmolL(-1) acetate buffer (pH 4.84) and were monitored by fluorescence detection using 1.5% 2-cyanoacetamide as a post-column fluorogenic reagent. As little as 50pmol of each uronate could be detected with excitation at 331nm and emission at 383nm.
Authors:
Akihisa Matsumoto; Saori Hosoyama; Kyohei Higashi; Toshihiko Toida
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-6
Journal Detail:
Title:  Carbohydrate research     Volume:  -     ISSN:  1873-426X     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.
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