Document Detail


Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection.
MedLine Citation:
PMID:  21612989     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An efficient and sensitive capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method has been developed for the simultaneous determination of phytohormones containing carboxyl group, including gibberellic acid, indole-3-acetic acid, abscisic acid, jasmonic acid, indole butyric acid, 1-naphthalene acetic acid and 2,4-dichloro-phenoxy acetic acid, based on the chemical derivatization with 6-oxy-(acetypiperazine) fluorescein. Using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the condensing reagent, the derivatization reaction completed at 60°C in 60min and the derivatization limits could reach 20nmolL(-1). The formed derivatives of seven phytohormones have been separated and quantified within 20min. The linearity was found in the range of 0.01-1μmolL(-1) and the limits of detection were 1.6-6.7nmolL(-1) (S/N=3). The proposed method has been applied to analyze the crude extract of 0.5g banana samples directly without further purification and the recoveries varying from 90.7 to 106.1%.
Authors:
Hao Chen; Xiao-Feng Guo; Hua-Shan Zhang; Hong Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-8
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  -     ISSN:  1873-376X     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
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