| Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection. | |
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MedLine Citation:
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PMID: 21612989 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An efficient and sensitive capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method has been developed for the simultaneous determination of phytohormones containing carboxyl group, including gibberellic acid, indole-3-acetic acid, abscisic acid, jasmonic acid, indole butyric acid, 1-naphthalene acetic acid and 2,4-dichloro-phenoxy acetic acid, based on the chemical derivatization with 6-oxy-(acetypiperazine) fluorescein. Using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the condensing reagent, the derivatization reaction completed at 60°C in 60min and the derivatization limits could reach 20nmolL(-1). The formed derivatives of seven phytohormones have been separated and quantified within 20min. The linearity was found in the range of 0.01-1μmolL(-1) and the limits of detection were 1.6-6.7nmolL(-1) (S/N=3). The proposed method has been applied to analyze the crude extract of 0.5g banana samples directly without further purification and the recoveries varying from 90.7 to 106.1%. |
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Authors:
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Hao Chen; Xiao-Feng Guo; Hua-Shan Zhang; Hong Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-8 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: - ISSN: 1873-376X ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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