| Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by HPLC coupled with diode array detection and evaporative light scattering detection. | |
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MedLine Citation:
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PMID: 20066678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A HPLC coupled with diode array detection (DAD) and evaporative light scattering detection (ELSD) method for qualitative and quantitative analysis of eight nucleosides and nucleobases, three carbohydrates and myriocin in Cordyceps was developed. A Prevail Carbohydrate ES column was employed for the separation within 50 min. Nucleosides and their bases were tested at UV 254 nm. ELSD was connected with DAD to determine myriocin and carbohydrates. The optimum drift tube temperature of ELSD was at 94 degrees C with the nitrogen flow rate of 2.0 L/min. All calibration curves showed good linearity (R(2)>0.9933) during the test ranges. The precision, repeatability, accuracy, LOD and LOQ were also fully investigated. This developed method was successfully applied to quantify 12 components, eight nucleosides and nucleobases, three carbohydrates and myriocin, in natural and cultured Cordyceps, which provides another view for quality control of Cordyceps sinensis. |
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Authors:
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Shuang Wang; Feng-Qing Yang; Kun Feng; De-Qiang Li; Jing Zhao; Shao-Ping Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Journal of separation science Volume: 32 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-20 Completed Date: 2010-03-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 4069-76 Citation Subset: IM |
Affiliation:
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Institute of Chinese Medical Sciences, University of Macau, Macao SAR, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrates
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analysis* Chromatography, High Pressure Liquid / methods* Cordyceps / chemistry* Drugs, Chinese Herbal / chemistry* Fatty Acids, Monounsaturated / analysis* Light Nucleosides / analysis* Plants, Medicinal / chemistry Scattering, Radiation |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Drugs, Chinese Herbal; 0/Fatty Acids, Monounsaturated; 0/Nucleosides; 35891-70-4/thermozymocidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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