Document Detail

Simultaneous determination of nine components in Qingkailing injection by HPLC/ELSD/DAD and its application to the quality control.
MedLine Citation:
PMID:  16257167     Owner:  NLM     Status:  MEDLINE    
High-performance liquid chromatography coupled with photo diode array detection and evaporative light scattering detection (HPLC/DAD/ELSD) was established to simultaneously determine nine ingredients in Qingkailing injection. Four wavelengths at 240, 254, 280 and 330 nm, respectively, were chosen as the monitoring wavelength to determine two nucleosides (uridine and adenosine), geniposide, baicalin and two organic acids (chlorogenic acid and caffeic acid), and an evaporative light scattering detector combined was employed to determine three steroids (cholic acid, ursodeoxycholic acid and hyodeoxycholic acid). This assay was fully validated in respect to precision, repeatability and accuracy. The proposed method was successfully applied to quantify the nine ingredients in 19 different Qingkailing injection samples and by principal component analysis (PCA) and hierarchical clustering analysis (HCA), it demonstrated significant variations in the content of these compounds in the samples from different manufacturers and preparation procedures. This method could be readily utilized as a quality control method for traditional Chinese medicine (TCM).
Shikai Yan; Guoan Luo; Yiming Wang; Yiyu Cheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-27
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  40     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2007-05-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  889-95     Citation Subset:  IM    
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, PR China.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Cluster Analysis
Drugs, Chinese Herbal / chemistry*
Principal Component Analysis
Quality Control
Reproducibility of Results
Scattering, Radiation
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Drugs, Chinese Herbal; 0/Qingkailing

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