Document Detail

Simultaneous determination of muscle 31P and O2 uptake kinetics during whole body NMR spectroscopy.
MedLine Citation:
PMID:  9931216     Owner:  NLM     Status:  MEDLINE    
Our understanding of O2 uptake (VO2) control mechanisms during exercise may be improved by the simultaneous determination of the kinetics of intramuscular high-energy phosphate turnover and pulmonary VO2. We therefore developed a technique for remote gas-exchange analysis while subjects exercised in a whole body 1.5-T NMR system. Knee-extension exercise was performed against restraining rubber bands in the prone position. Free induction decays were acquired every 1,875 ms by using a transmit-receive coil, which was placed under the quadriceps. This allowed 31P spectra of intramuscular ATP, Pi, and creatine phosphate dynamics to be determined every 15 s. Airflow was measured with a custom-designed turbine and a 45-ft.-long cable to reach the volume-measuring module. This was located in an adjacent radio-frequency-shielded room, as was the respiratory mass spectrometer, which also used a 45-ft.-long sampling line. The respired gas profiles were not discernibly different from those that used the standard inlet; the increase in the delay was readily incorporated into the breathby-breath algorithm, allowing the VO2 kinetics to be determined in concert with those of intramuscular phosphate metabolism.
B J Whipp; H B Rossiter; S A Ward; D Avery; V L Doyle; F A Howe; J R Griffiths
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  86     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-22     Completed Date:  1999-03-22     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  742-7     Citation Subset:  IM    
Department of Physiology, University of Glasgow, United Kingdom.
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MeSH Terms
Adenosine Triphosphate / metabolism
Carbon Dioxide / analysis,  blood
Exercise / physiology
Magnetic Resonance Spectroscopy
Muscle, Skeletal / chemistry*,  metabolism*
Oxygen / analysis,  metabolism*
Oxygen Consumption / physiology*
Phosphates / analysis
Phosphocreatine / chemistry,  metabolism
Phosphorus / analysis,  metabolism*
Phosphorus Isotopes
Respiratory Function Tests
Reg. No./Substance:
0/Phosphates; 0/Phosphorus Isotopes; 124-38-9/Carbon Dioxide; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine; 7723-14-0/Phosphorus; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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