| Simultaneous determination of muscle 31P and O2 uptake kinetics during whole body NMR spectroscopy. | |
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MedLine Citation:
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PMID: 9931216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our understanding of O2 uptake (VO2) control mechanisms during exercise may be improved by the simultaneous determination of the kinetics of intramuscular high-energy phosphate turnover and pulmonary VO2. We therefore developed a technique for remote gas-exchange analysis while subjects exercised in a whole body 1.5-T NMR system. Knee-extension exercise was performed against restraining rubber bands in the prone position. Free induction decays were acquired every 1,875 ms by using a transmit-receive coil, which was placed under the quadriceps. This allowed 31P spectra of intramuscular ATP, Pi, and creatine phosphate dynamics to be determined every 15 s. Airflow was measured with a custom-designed turbine and a 45-ft.-long cable to reach the volume-measuring module. This was located in an adjacent radio-frequency-shielded room, as was the respiratory mass spectrometer, which also used a 45-ft.-long sampling line. The respired gas profiles were not discernibly different from those that used the standard inlet; the increase in the delay was readily incorporated into the breathby-breath algorithm, allowing the VO2 kinetics to be determined in concert with those of intramuscular phosphate metabolism. |
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Authors:
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B J Whipp; H B Rossiter; S A Ward; D Avery; V L Doyle; F A Howe; J R Griffiths |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 86 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-03-22 Completed Date: 1999-03-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 742-7 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Glasgow, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Carbon Dioxide / analysis, blood Exercise / physiology Humans Kinetics Magnetic Resonance Spectroscopy Muscle, Skeletal / chemistry*, metabolism* Oxygen / analysis, metabolism* Oxygen Consumption / physiology* Phosphates / analysis Phosphocreatine / chemistry, metabolism Phosphorus / analysis, metabolism* Phosphorus Isotopes Respiratory Function Tests |
| Chemical | |
Reg. No./Substance:
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0/Phosphates; 0/Phosphorus Isotopes; 124-38-9/Carbon Dioxide; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine; 7723-14-0/Phosphorus; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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