Document Detail

Simultaneous determination of metoprolol and α-hydroxymetoprolol in human plasma using excitation-emission matrix fluorescence coupled with second-order calibration methods.
MedLine Citation:
PMID:  23216119     Owner:  NLM     Status:  In-Data-Review    
Background: Metoprolol (MET) is a β1-adrenoceptor antagonist, which is widely used in the treatment of cardiovascular diseases, and α-hydroxymetoprolol (α-OHM) is its hydroxylated metabolite. Owing to their similar structures, optimization of the condition for the chromatography approach, which is in common use for determination, is both time consuming and laborious. Results: A new and effective strategy that combines the excitation-emission matrix fluorescence with second-order calibration methods was developed for simultaneous determination of MET and α-OHM in human plasma. Conclusion: Although the fluorescence spectra of MET and α-OHM overlapped and a large number of unknown and uncalibrated fluorescent components coexisted, the developed method enables accurate concentrations together with reasonable resolution of excitation and emission profiles for the analytes of interest. An additional advantage of the proposed method is that there is no need for separation and sample pretreatment, in addition to lower cost than traditional methods.
Hui-Wen Gu; Hai-Long Wu; Ya-Juan Liu; Xiao-Li Yin; Zhi-Yi Su; Shan-Shan Li; Ru-Qin Yu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  4     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  2781-93     Citation Subset:  IM    
State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
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