Document Detail

Simultaneous determination of metformin in combination with rosiglitazone by reversed-phase liquid chromatography.
MedLine Citation:
PMID:  15023258     Owner:  NLM     Status:  MEDLINE    
A simple, rapid, and precise reversed-phase liquid chromatographic method is developed for the simultaneous determination of metformin in combination with rosiglitazone. This method uses a Zorbax XDB C(18) 15-cm analytical column, a mobile phase of acetonitrile and buffer containing 10mM disodium hydrogen phsosphate, and 5mM sodium dodecyl sulphate in the ratio of 34:66 (v/v), and pH is adjusted to 7.1 with orthophosphoric acid. The instrumental settings are a flow rate of 1 mL/min, column temperature at 40 degrees C, and detector wavelength of 226 nm. The internal standard method is used for the quantitation of metformin and rosiglitazone. Methylparaben is used as an internal standard. The method is validated and shown to be linear. The correlation coefficients for metformin and rosiglitazone are 0.9996 and 0.9997, respectively. The relative standard deviation for six replicate measurements in two sets of each drug in the tablets is always less than 2%.
B L Kolte; B B Raut; A A Deo; M A Bagool; D B Shinde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  42     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-16     Completed Date:  2004-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  70-3     Citation Subset:  IM    
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra State, India.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Metformin / analysis*
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Thiazolidinediones / analysis*
Reg. No./Substance:
0/Thiazolidinediones; 122320-73-4/rosiglitazone; 657-24-9/Metformin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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