Document Detail

Simultaneous determination of iridoids, phenolic acids, flavonoids, and saponins in Flos Lonicerae and Flos Lonicerae Japonicae by HPLC-DAD-ELSD coupled with principal component analysis.
MedLine Citation:
PMID:  18069743     Owner:  NLM     Status:  MEDLINE    
A method, HPLC coupled with diode-array and evaporative light scattering detectors (HPLC-DAD-ELSD), was newly developed to evaluate the quality of Flos Lonicerae (FL) and Flos Lonicerae Japonicae (FLJ), through a simultaneous determination of multiple types of bioactive components. By employing DAD, the detection wavelengths were set at 240 nm for the determination of iridoids, 330 nm for phenolic acids, and 360 nm for flavonoids, respectively. While ELSD, connected in series after DAD, was applied to the determination of saponins. This assay was fully validated with respect to precision, repeatability, and accuracy. Moreover, principal component analysis (PCA) was used for the similarity evaluation of different samples, and it was proven straightforward and reliable to differentiate FL and FLJ samples from different origins. For PCA, two principal components have been extracted. Principal component 1 (PC1) influences the separation between different sample sets, capturing 54.598% variance, while principal component 2 (PC2) affects differentiation within sample sets, capturing 12.579% variance. In conclusion, simultaneous quantification of bioactive components by HPLC-DAD-ELSD coupled with PCA would be a well-acceptable strategy to differentiate the sources and to comprehensively control the quality of the medicinal plants FL and FLJ.
Chun-Yun Chen; Lian-Wen Qi; Hui-Jun Li; Ping Li; Ling Yi; Hong-Liang Ma; Dan Tang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  30     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3181-92     Citation Subset:  IM    
Key Laboratory of Modern Chinese Medicines, Ministry of Education, China Pharmaceutical University, Nanjing, People's Republic of China.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Flavonoids / analysis*,  chemistry
Gases / chemistry*
Hydroxybenzoic Acids / analysis*,  chemistry
Iridoids / analysis*,  chemistry
Lepidoptera / chemistry*
Molecular Structure
Saponins / analysis*,  chemistry
Reg. No./Substance:
0/Flavonoids; 0/Gases; 0/Hydroxybenzoic Acids; 0/Iridoids; 0/Saponins; 29656-58-4/phenolic acid

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